Original Articles
Copyright ©2009 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. May 28, 2009; 15(20): 2500-2505
Published online May 28, 2009. doi: 10.3748/wjg.15.2500
Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide
Ye Zong, Shu-Tian Zhang, Sheng-Tao Zhu
Ye Zong, Shu-Tian Zhang, Sheng-Tao Zhu, Department of Gastroenterology, Beijing Friendship Hospital Affiliated to the Capital Medical University, Beijing Digestive Disease Center, Beijing 100050, China
Author contributions: Zhang ST, Zong Y contributed equally to this work; Zhang ST, Zong Y designed the research; Zong Y, Zhu ST performed the research and analyzed the data; Zong Y, Zhang ST wrote the paper.
Correspondence to: Shu-Tian Zhang, Department of Gastroenterology, Beijing Friendship Hospital affiliated to the Capital Medical University, Beijing Digestive Disease Center, Beijing 100050, China. zhangst@bddc-bfh.com.cn
Telephone: +86-10-63138702
Fax: +86-10-63138339
Received: February 5, 2009
Revised: March 28, 2009
Accepted: April 4, 2009
Published online: May 28, 2009
Abstract

AIM: To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.

METHODS: The esophageal squamous carcinoma cell line (TE-13) was treated with different concentrations of nicotine (100 &mgr;g/mL and 200 &mgr;g/mL) or 200 &mgr;g/mL nicotine plus 100 &mgr;mol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 expression was determined by Western blotting. Matrix metalloproteinase-2 (MMP-2) was analyzed by zymography and ELISA.

RESULTS: Nicotine (100 &mgr;g/mL, 200 &mgr;g/mL) enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotine (200 &mgr;g/mL) stimulated TE-13 cells migration and invasion which were partly blocked by nimesulide. This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2.

CONCLUSION: Nicotine enhances the migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect of nicotine-enhanced esophageal squamous carcinoma cell migration and invasion.

Keywords: Carcinoma; Cyclooxygenase 2 inhibitors; Esophagus; Nicotine; Squamous cell