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World J Gastroenterol. Mar 28, 2009; 15(12): 1528-1529
Published online Mar 28, 2009. doi: 10.3748/wjg.15.1528
Published online Mar 28, 2009. doi: 10.3748/wjg.15.1528
Successful isolation of Helicobacter pylori after prolonged incubation from a patient with failed eradication therapy
Yan Yin, Li-Hua He, Jian-Zhong Zhang, Department of Diagnosis, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Author contributions: Yin Y, Zhang JZ and He LH designed the research; Yin Y and He LH performed the research; Yin Y, He LH and Zhang JZ analyzed the data; Yin Y and Zhang JZ wrote the paper.
Correspondence to: Jian-Zhong Zhang, Professor, Department of Diagnosis, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China. zhangjianzhong@icdc.cn
Telephone: +86-10-61739319
Fax: +86-10-61730233
Received: January 6, 2008
Revised: February 20, 2009
Accepted: February 27, 2009
Published online: March 28, 2009
Revised: February 20, 2009
Accepted: February 27, 2009
Published online: March 28, 2009
Abstract
Helicobacter pylori (H pylori), a gastric pathogen, is a major cause of chronic gastritis and peptic ulcer disease, and is an important risk factor for the development of gastric malignancies. Culture of the bacterium from gastric biopsy is essential for the determination of drug resistance of H pylori. However, the isolation rates of H pylori from infected individuals vary from 23.5% to 97% due to a number of factors such as biopsy preparation, cultural environment, medium and the method adopted. In the present case, we found that a prolonged incubation period of up to 19 d allowed successful isolation of H pylori from a patient who received triple therapy that failed to eradicate the bacterium.
Keywords: Helicobacter pylori; Isolation; Eradication