Yan YG, Zhao G, Ma JP, Cai SR, Zhan WH. Effects of different Helicobacter pylori culture filtrates on growth of gastric epithelial cells. World J Gastroenterol 2008; 14(23): 3745-3749 [PMID: 18595143 DOI: 10.3748/wjg.14.3745]
Corresponding Author of This Article
Yan-Guo Yan, Department of General Surgery, The Affiliated Hospital of Wuhan University of Science & Technology, Wuhan 430064, Hubei Province, China. yan969400@yahoo.com.cn
Article-Type of This Article
Rapid Communication
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World J Gastroenterol. Jun 21, 2008; 14(23): 3745-3749 Published online Jun 21, 2008. doi: 10.3748/wjg.14.3745
Effects of different Helicobacter pylori culture filtrates on growth of gastric epithelial cells
Yan-Guo Yan, Gang Zhao, Jin-Ping Ma, Shi-Rong Cai, Wen-Hua Zhan
Yan-Guo Yan, Department of General Surgery, The Affiliated Hospital of Wuhan University of Science & Technology, Wuhan 430064, Hubei Province, China
Gang Zhao, The Second Department of General Surgery, Guangdong Province Hospital, Guangzhou 510080, Guangdong Province, China
Jin-Ping Ma, Shi-Rong Cai, Wen-Hua Zhan, Department of Gastrointestinal and Pancreatic Surgery, The First Affiliated Hospital, Research Center for Gastric Cancer, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
Author contributions: Yan YG, Zhao G, performed the research and wrote the paper and contributed equally to this work; Zhan WH and Ma JP designed the research; Cai SR recorded the data.
Correspondence to: Yan-Guo Yan, Department of General Surgery, The Affiliated Hospital of Wuhan University of Science & Technology, Wuhan 430064, Hubei Province, China. yan969400@yahoo.com.cn
Telephone: +86-27-51164927
Fax: +86-27-51163527
Received: January 6, 2008 Revised: April 21, 2008 Accepted: April 28, 2008 Published online: June 21, 2008
Abstract
AIM: To study the effects of different Helicobacter pylori (H pylori) culture filtrates on growth of gastric epithelial cells.
METHODS: Broth culture filtrates of H pylori were prepared. Gastric epithelial cells were treated with the filtrates, and cell growth was determined by growth curve and flow cytometry. DNA damage of gastric epithelial cells was measured by single-cell microgel electrophoresis.
RESULTS: Gastric epithelial cells proliferated actively when treated by CagA-gene-positive broth culture filtrates, and colony formation reached 40%. The number of cells in S phase increased compared to controls. Comet assay showed 41.2% comet cells in GES-1 cells treated with CagA-positive filtrates (P < 0.05).
CONCLUSION: CagA-positive filtrates enhance the changes in morphology and growth characteristics of human gastric epithelial tumor cells. DNA damage maybe one of the mechanisms involved in the growth changes.
