Rapid Communication
Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Apr 28, 2008; 14(16): 2578-2581
Published online Apr 28, 2008. doi: 10.3748/wjg.14.2578
Effect of histone deacetylase inhibitor on proliferation of biliary tract cancer cell lines
Li-Ning Xu, Xin Wang, Sheng-Quan Zou
Li-Ning Xu, Institute of Hepatobiliary Surgery of PLA General Hospital, Beijing 100853, China
Xin Wang, Sheng-Quan Zou, Department of General Surgery of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Author contributions: Xu LN and Wang X contributed equally to this work; Xu LN and Wang X designed the research; Xu LN, Wang X and Zou SQ performed the research; Wang X and Zou SQ analyzed the data; Xu LN wrote the paper.
Correspondence to: Li-Ning Xu, Institute of Hepatobiliary Surgery of PLA General Hospital, Beijing 100853, China. chaoyue528@sohu.com
Telephone: +86-10-66936609
Fax: +86-10-66936609
Received: December 9, 2007
Revised: March 4, 2008
Published online: April 28, 2008
Abstract

AIM: To explore the effect of histone deacetylase inhibitor, trichostatin A (TSA) on the growth of biliary tract cancer cell lines (gallbladder carcinoma cell line and cholangiocarcinoma cell line) in vivo and in vitro, and to investigate the perspective of histone deacetylase inhibitor in its clinical application.

METHODS: The survival rates of gallbladder carcinoma cell line (Mz-ChA-l cell line) and cholangiocarcinoma cell lines (QBC939, KMBC and OZ cell lines) treated with various doses of TSA were detected by methylthiazol tetrazolium (MTT) assay. A nude mouse model of transplanted gallbladder carcinoma (Mz-ChA-l cell line) was successfully established, and changes in the growth of transplanted tumor after treated with TSA were measured.

RESULTS: TSA could inhibit the proliferation of gallbladder carcinoma cell line (Mz-ChA-l cell line) and cholangiocarcinoma cell lines (QBC939, KMBC and OZ cell lines) in a dose-dependent manner. After the nude mouse model of transplanted gallbladder carcinoma (Mz-ChA-l cell line) was successfully established, the growth of cancer was inhibited in the model after treated with TSA.

CONCLUSION: TSA can inhibit the growth of cholangiocarcinoma and gallbladder carcinoma cell lines in vitro and in vivo.

Keywords: Biliary tract cancer; Gallbladder carcinoma; Cholangiocarcinoma; Proliferation; Trichostatin A