Basic Research
Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Apr 28, 2008; 14(16): 2494-2500
Published online Apr 28, 2008. doi: 10.3748/wjg.14.2494
Effect of 5-LOX/COX-2 common inhibitor DHDMBF30 on pancreatic cancer cell Capan2
Bo Zhang, Chang-Liang Wang, Wen-Hua Zhao, Ming Lv, Chun-Ying Wang, Wei-Xia Zhong, Wu-Yuan Zhou, Wen-Sheng Yu, Yan Zhang, Sheng Li
Bo Zhang, Chang-Liang Wang, Wen-Hua Zhao, Chun-Ying Wang, Wei-Xia Zhong, Wu-Yuan Zhou, Wen-Sheng Yu, Sheng Li, Shandong Tumor Hospital, Jinan 250117, Shandong Province, China
Ming Lv, Zibo central hospital, Zibo 255001, Shandong Province, China
Yan Zhang, Qilu hospital of Shandong University, Jinan 250011, Shandong Province, China
Author contributions: Zhang B, Wang CL and Zhang Y contributed equally to this work.
Correspondence to: Sheng Li, Shandong Tumor Hospital, Jinan 250117, Shandong Province, China. drlisheng@sohu.com
Telephone: +86-13705311949
Fax: +86-531-87984079
Received: November 13, 2007
Revised: December 11, 2007
Published online: April 28, 2008
Abstract

AIM: To study the effect of 5-lipoxygenase/cyclooxy-genase-2 (5-LOX/COX-2) dual inhibitor 7-tert-butyl-2, 3-dihydro-3, 3-dimethyl substituted dihydrofuran 30 (DHDMBF30) on proliferation and apoptosis of the pancreatic cancer cell line Capan-2 and the effect of DHDMBF30 on human pancreatic cancer in a nude mouse model.

METHODS: Investigate the effect of 5-LOX/COX-2 dual inhibitor DHDMBF30 on proliferation and apoptosis of the pancreatic cancer cell line Capan-2 by RT-PCR, MTT assay, FCM and electron microscope. Cell line Capan-2 was inoculated percutaneously on the outer thigh of 12 nude mice. The VEGF mRNA of transplantation tumor was detected by RT-PCR.

RESULTS: DHDMBF30 inhibits the proliferation of cell line Capan2, reduces the expression of 5-LOX, COX-2 and VEGF. After Capan2 was treated with DHDMBF30, we found that the apoptosis peak of the experimental group was significantly higher than that of the contrast group (3.08 ± 1.89 vs 27.67 ± 0.52, P < 0.001). The tumor weight of the DHDMBF30 group was significantly lower than PBS control groups (1.35 ± 0.47 vs 2.92 ± 0.73, P < 0.01). Expression of VEGF in the DHDMBF30 group was significantly decreased.

CONCLUSION: DHDMBF30 inhibits the proliferation of the pancreatic cell line Capan2, and induces apoptosis and inhibits the growth of pancreatic cancer in nude mice.

Keywords: 5-lipoxygenase pancreatic tumor Cycloo-xygenase2; DHDMBF30; Carcinoma in nude mice