Chen S, Cheng AC, Wang MS, Peng X. Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling. World J Gastroenterol 2008; 14(14): 2174-2178 [PMID: 18407590 DOI: 10.3748/wjg.14.2174]
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Professor An-Chun Cheng, Avian Diseases Research Centre, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China. chenganchun@vip.163.com
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Chen S, Cheng AC, Wang MS, Peng X. Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling. World J Gastroenterol 2008; 14(14): 2174-2178 [PMID: 18407590 DOI: 10.3748/wjg.14.2174]
World J Gastroenterol. Apr 14, 2008; 14(14): 2174-2178 Published online Apr 14, 2008. doi: 10.3748/wjg.14.2174
Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling
Shun Chen, An-Chun Cheng, Ming-Shu Wang, Xi Peng
Shun Chen, Avian Diseases Research Centre, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China
An-Chun Cheng, Ming-Shu Wang, Xi Peng, Avian Diseases Research Centre, College of Veterinary Medicine of Sichuan Agricultural University; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Yaan 625014, Sichuan Province, China
Ming-Shu Wang, College of Life Science and Technology of Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China
Author contributions: Chen S, Cheng AC and Wang MS contributed equally to this work and designed the research; Chen S performed the research, analyzed data and wrote the paper; Peng X provided analytic tools.
Correspondence to: Professor An-Chun Cheng, Avian Diseases Research Centre, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China. chenganchun@vip.163.com
Telephone: +86-835-2885774
Fax: +86-835-2885774
Received: December 5, 2007 Revised: January 17, 2008 Published online: April 14, 2008
Abstract
AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells.
METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI).
RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non-apoptotic cells decreased.
CONCLUSION: NGVEV can induce the infected DEF cells to undergo apoptosis and the apoptosis occurs prior to necrosis.
