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World J Gastroenterol. Oct 14, 2007; 13(38): 5139-5146
Published online Oct 14, 2007. doi: 10.3748/wjg.v13.i38.5139
Pretreatment of cromolyn sodium prior to reperfusion attenuates early reperfusion injury after the small intestine ischemia in rats
Zi-Qing Hei, Xiao-Liang Gan, Gang-Jian Luo, Shang-Rong Li, Jun Cai
Zi-Qing Hei, Xiao-Liang Gan, Gang-Jian Luo, Shang-Rong Li, Jun Cai, Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Supported by The Chinese Traditional Medicine Foundation of Guangdong Province, China, No. 1040051
Correspondence to: Dr. Zi-Qing Hei, Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China. heiziqing0530@hotmail.com
Telephone: +86-20-87580867
Received: June 24, 2007
Revised: July 26, 2007
Accepted: August 6, 2007
Published online: October 14, 2007
Abstract

AIM: To investigate the effects of Cromolyn Sodium (CS) pretreated prior to reperfusion on the activity of intestinal mucosal mast cells (IMMC) and mucous membrane of the small intestine in ischemia-reperfusion (IR) injury of rats.

METHODS: Thirty-two Sprague-Dawley (SD) rats were randomly divided into four groups: sham group (group S), model group (group M), high and low dosage of CS groups, (treated with CS 50 mg/kg or 25 mg/kg, group C1 and C2). Intestinal IR damage was induced by clamping the superior mesenteric artery for 45 min followed by reperfusion for 60 min. CS was intravenouly administrated 15 min before reperfusion. Ultrastructure and counts of IMMC, intestinal structure, the expression of tryptase, levels of malondisldehyde (MDA), TNF-α, histamine and superoxide dismutase (SOD) activity of the small intestine were detected at the end of experiment.

RESULTS: The degranulation of IMMC was seen in group M and was attenuated by CS treatment. Chiu’s score of group M was higher than the other groups. CS could attenuate the up-regulation of the Chiu’s score, the levels of MDA, TNF-α, and expression of tryptase and the down-regulation of SOD activity and histamine concentration. The Chiu’s score and MDA content were negatively correlated, while SOD activity was positively correlated to the histamine concentration respectively in the IR groups.

CONCLUSION: Pretreated of CS prior to reperfusion protects the small intestine mucous from ischemia-reperfusion damage, the mechanism is inhibited IMMC from degranulation.

Keywords: Ischemia; Reperfusion injury; Intestinal mucosal mast cells; Histamine; Tumor necrosis factor-α