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Copyright ©2007 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Oct 7, 2007; 13(37): 4960-4966
Published online Oct 7, 2007. doi: 10.3748/wjg.v13.i37.4960
Effect of ethanol on pro-apoptotic mechanisms in polarized hepatic cells
Benita L McVicker, Dean J Tuma, Carol A Casey
Benita L McVicker, Dean J Tuma, Carol A Casey, Liver Study Unit, Department of Veterans Affairs Medical Center; and Departments of Internal Medicine and Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68105, United States
Author contributions: All authors contributed equally to the work.
Supported by the National Institute on Alcohol Abuse and Alcoholism and the Department of Veterans Affairs
Correspondence to: Benita L McVicker, PhD, Veterans Affairs Medical Center, Research Service (151), 4101 Woolworth Avenue, Omaha, NE 68105, United States. bmcvicker@unmc.edu
Telephone: +1-402-3468800-3547 Fax: +1-402-4490604
Received: June 30, 2007
Revised: July 18, 2007
Accepted: July 26, 2007
Published online: October 7, 2007
Abstract

Chronic ethanol consumption is associated with serious and potentially fatal alcohol-related liver injuries such as hepatomegaly, alcoholic hepatitis and cirrhosis. Moreover, it has been documented that the clinical progression of alcohol-induced liver damage may be associated with an increase in hepatocellular death that involves apoptotic mechanisms. Although much information has been learned about the clinical manifestations associated with alcohol-related diseases, the search continues for a better understanding of the molecular and/or cellular mechanisms by which ethanol exerts its deleterious effects such as the induction of pro-apoptotic mechanisms and related cell damaging events. As part of the effort to enhance our understanding of those particular cellular pathways and mechanisms associated with ethanol toxicity, researchers over the years have utilized a variety of model systems. Recently, work has come forth demonstrating the utility of a hybrid cell line (WIF-B) as a cell culture model system for the study of alcohol-associated alterations in hepatocellular mechanisms. Success with such emerging model systems could aid in the development of potential therapeutic treatments for the prevention of alcohol-induced apoptotic cell death that may ultimately serve as a significant target in delaying the onset and/or progression of clinical symptoms of alcohol-mediated liver disease. This review article summarizes the current understanding of ethanol-mediated modifications in cell survival and thus the promotion of pro-apoptotic events with emphasis on analyses made in various experimental model systems, particularly the more recently characterized WIF-B cell system.

Keywords: Hepatoma hybrid cells; Ethanol; Apoptosis; Fas receptor; Caspase