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World J Gastroenterol. Jul 21, 2007; 13(27): 3742-3746
Published online Jul 21, 2007. doi: 10.3748/wjg.v13.i27.3742
Cross-reactivity of anti-H pylori antibodies with membrane antigens of human erythrocytes
Feng-Hua Guo, Xiao-Mei Yan, Chun-Xiang Fan, Fei Zhao, Yuan Hu, Di Xiao, Xun Zeng, Mao-Jun Zhang, Li-Hua He, Fan-Ling Meng, Jian-Zhong Zhang
Feng-Hua Guo, Xiao-Mei Yan, Chun-Xiang Fan, Fei Zhao, Yuan Hu, Di Xiao, Xun Zeng, Mao-Jun Zhang, Li-Hua He, Fan-Ling Meng, Jian-Zhong Zhang, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, PO Box 5, Changping, Beijing 102206, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30370078
Correspondence to: Jian-Zhong Zhang, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, PO Box 5, Changping, Beijing 102206, China. helico99@sina.com
Telephone: +86-10-61739456 Fax: +86-10-61739456
Received: March 10, 2007
Revised: April 10, 2007
Accepted: April 18, 2007
Published online: July 21, 2007
Abstract

AIM: To investigate whether anti-H pylori antibodies have cross-reaction with antigens of erythrocyte membrane.

METHODS: Blood samples were collected from 14 volunteers (8 positive and 6 negative for H pylori detected by 13C-urea breath test) of the general population. Erythrocyte membrane proteins of the subjects were examined by Western blot using anti-H pylori serum. The proteins related to the positive bands were identified by mass spectrum analysis.

RESULTS: Anti-H pylori antibodies had cross-reaction with the proteins of about 50 kDa of erythrocyte membranes in all samples independent of H pylori infection. One protein in the positive band was identified as Chain S, the crystal structure of the cytoplasmic domain of human erythrocyte Band-3 protein.

CONCLUSION: Anti-H pylori antibodies cross-react with some antigens of human erythrocyte membrane, which may provide a clue for the relationship between H pylori infection and vascular disorders.

Keywords: H pylori; Antibodies; Erythrocyte; Cross-reactivity