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World J Gastroenterol. May 28, 2007; 13(20): 2858-2861
Published online May 28, 2007. doi: 10.3748/wjg.v13.i20.2858
Clinical features of familial adenomas polyps in Chinese and establishment of its immortal lymphocyte cell lines
Shan-Rong Cai, Su-Zhang Zhang, Shu Zheng
Shan-Rong Cai, Su-Zhang Zhang, Shu Zheng, Cancer Institute, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by National 863 Program of China, No. 2004AA227070
Correspondence to: Dr Shu Zheng, Cancer Institute, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China. caisr2007@yahoo.com.cn
Telephone: +86-571-87784528 Fax: +86-571-87214404
Received: January 19, 2007
Revised: February 25, 2007
Accepted: March 1, 2007
Published online: May 28, 2007
Abstract

AIM: To reserve the rare Chinese familial adenomas polyp (FAP) family resource and to investigate the clinical features of FAP in Chinese for its diagnosis.

METHODS: Clinical features of patients with FAP were investigated. If there is any question, their medical records were verified. Blood sample was taken and lymphocyte immortal cell lines were established with modified EB-transformation methods. Congenital hypertrophy of retinal pigment epithelium (CHRPE) was checked by an experienced ophthalmologist.

RESULTS: Twenty seven families including 21 classical FAP (CFAP) families, 3 attenuated FAP (AFAP) families, and 3 suspected AFAP families were investigated. A total of 116 lymphocyte immortal cell lines were established from 26 families. In all the FAP families, colorectal cancer occurred at the mean age of 42.84 years. Of the 16 families checked, 15 (93.75%) had CHRPE. The mean number of patients suffering from colorectal neoplasm was 3.14 in CFAP families and 2.0 in AFAP families (P < 0.01). The mean oldest age at diagnosis of FAP was 41.75 years in CFAP families, and 58.67 years in AFAP families, respectively (P < 0.01). Mean age of development of colorectal cancer was 42.23 in CFAP and 57.33 years old in AFAP (P < 0.01). Mean of the earliest age at diagnosis of FAP was 29.95 years in the FAP families with a positive family history and 46.80 years in the FAP families with a negative family history (P < 0.01). The ratio of extra-intestinal tumors to colorectal neoplasms was different in the two kinds of families with positive and negative family history (P < 0.01).

CONCLUSION: Additional use of ciclosporin will effectively improve to establish lymphocyte immortal cell lines with modified EB- transformation methods. In Chinese FAP, there was a high frequency of CHRPE , and a later age at diagnosis and a later age of development of colorectal cancer in AFAP. And earlier age at diagnosis in FAP with positive family history was also found that will help to diagnose various kinds of FAP in Chinese.

Keywords: Adenomatous polyposis coli; Pedigree; Phenotype; Family history; Congenital hypertrophy of retinal pigment epithelium; Immortal cell line