Detection of carcinoembryonic antigen mRNA in peritoneal washes from gastric cancer patients and its clinical significance

doi:10.3748/wjg.v12.i9.1408

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Mar 7, 2006 (publication date) through Nov 3, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 7, 2006; 12(9): 1408-1411
Published online Mar 7, 2006. doi: 10.3748/wjg.v12.i9.1408

Detection of carcinoembryonic antigen mRNA in peritoneal washes from gastric cancer patients and its clinical significance

Yan-Song Zhang, Jun Xu, Guang-Hua Luo, Rong-Chao Wang, Jiang Zhu, Xiao-Ying Zhang, Peter Nilsson-Ehle, Ning Xu

Yan-Song Zhang, Jun Xu, Rong-Chao Wang, Department of Gastrointestinal Surgery, the Third Affiliated Hospital of Suzhou University, Changzhou 213003, Jiangsu Province, China

Guang-Hua Luo, Jiang Zhu, Laboratory of Molecular Biology, the Third Affiliated Hospital of Suzhou University, Changzhou 213003, Jiangsu Province, China

Xiao-Ying Zhang, Department of Thoracic Surgery, the Third Affiliated Hospital of Suzhou University, Changzhou 213003, Jiangsu Province, China

Peter Nilsson-Ehle, Ning Xu, Department of Clinical Chemistry, Institute of Laboratory Medicine, University Hospital of Lund, Lund University, Lund 221 85, Sweden

Correspondence to: Ning Xu, MD, PhD, Department of Clinical Chemistry, Institute of Laboratory Medicine, University Hospital of Lund, Lund S-221 85, Sweden. ning.xu@klinkem.lu.se

Telephone: +46-46-173462 Fax: +46-46-130064

Received: June 29, 2005
Revised: August 1, 2005
Accepted: October 9, 2005
Published online: March 7, 2006

Abstract

AIM: To establish a more sensitive method for detection of free cancer cells in peritoneal washes from gastric cancer patients during surgery and to evaluate its clinical significance.

METHODS: The carcinoembryonic antigen (CEA) mRNA levels in peritoneal washes from 65 cases of gastric cancer were detected by real-time RT-PCR. Peritoneal lavage cytology (PLC) was applied simultaneously to detection of free cancer cells. Negative controls included peritoneal washes from 5 cases of benign gastric disease and blood samples from 5 adult healthy volunteers.

RESULTS: There was no CEA mRNA in peritoneal washes from benign gastric disease patients and in blood of adult healthy volunteers. The positive percentage of free cancer cells detected by real-time RT-PCR was 47.7% and only 12.3% by PLC. The positive rate of CEA mRNA was significantly related with serosa invasion between peritoneal metastasis and stage of gastric cancer.

CONCLUSION: Real-time RT-PCR is a sensitive and rapid method for the detection of free cancer cells in peritoneal washes. The presence of free cancer cells in peritoneal washes is related to the pathologic stage of gastric cancer.

Keywords: Real-time RT-PCR; Peritoneal washes; Gastric cancer; Carcinoembryonic antigen