Pastor CM, Vonlaufen A, Georgi F, Hadengue A, Morel P, Frossard JL. Neutrophil depletion-but not prevention of Kupffer cell activation-decreases the severity of cerulein-induced acute pancreatitis. World J Gastroenterol 2006; 12(8): 1219-1224 [PMID: 16534874 DOI: 10.3748/wjg.v12.i8.1219]
Corresponding Author of This Article
Dr Catherine M Pastor, Laboratoire de Physiopathologie Hépatique et Imagerie Moléculaire, Hôpitaux Universitaires de Genève, Bâtiment C, Room 6-795, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland. catherine.pastor@hcuge.ch
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Basic Research
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World J Gastroenterol. Feb 28, 2006; 12(8): 1219-1224 Published online Feb 28, 2006. doi: 10.3748/wjg.v12.i8.1219
Neutrophil depletion-but not prevention of Kupffer cell activation-decreases the severity of cerulein-induced acute pancreatitis
Catherine M Pastor, Alain Vonlaufen, Fabianna Georgi, Antoine Hadengue, Philippe Morel, Jean-Louis Frossard
Catherine M Pastor, Laboratoire de Physiopathologie Hépatique et Imagerie Moléculaire, Hôpitaux Universitaires de Genève, Bâtiment C, Room 6-795, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Alain Vonlaufen, Division of Gastroentérologie et Hépatologie, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Fabianna Georgi, Département APSI, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Antoine Hadengue, Division of Gastroentérologie et Hépatologie, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Philippe Morel, Département de Chirurgie, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Jean-Louis Frossard, Division of Gastroentérologie et Hépatologie, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Author contributions: All authors contributed equally to the work.
Supported by a grant from the Fonds National Suisse de la Recherche Scientifique N° 3200B0-100764 to Jean-Louis Frossard
Correspondence to: Dr Catherine M Pastor, Laboratoire de Physiopathologie Hépatique et Imagerie Moléculaire, Hôpitaux Universitaires de Genève, Bâtiment C, Room 6-795, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland. catherine.pastor@hcuge.ch
Telephone: +41-22-3729353 Fax: +41-22-3729366
Received: June 30, 2005 Revised: July 7, 2005 Accepted: July 15, 2005 Published online: February 28, 2006
Abstract
AIM: To determine whether neutrophil depletion and Kupffer cell inhibition might combine their protective effects to decrease the severity of acute pancreatitis.
METHODS: Mice had cerulein administration to induce acute pancreatitis and were pretreated with either anti-mouse neutrophil serum or gadolinium chloride (GdCl3) to prevent Kupffer cell activation, or both treatments. Injury was assessed in pancreas and lungs. Myeloperoxidases (MPO) assessed neutrophil infiltration. Interleukin-6 (IL-6) and IL-10 were measured in serum, pancreas, lungs and liver.
RESULTS: In mice with acute pancreatitis, neutrophil depletion reduced the severity of pancreatitis and pancreatitis-associated lung injury. Kupffer cell inactivation by GdCl3 had less protective effect, although IL-6 and IL-10 concentrations were significantly decreased. The protective treatment brought by neutrophil depletion was not enhanced by Kupffer cell inactivation and both treatments did not combine their protective effects.
CONCLUSION: Our results confirm the role of activated neutrophils in aggravating organ injury in acute pancreatitis while the role of Kupffer cell activation is less obvious.
Pastor CM, Vonlaufen A, Georgi F, Hadengue A, Morel P, Frossard JL. Neutrophil depletion-but not prevention of Kupffer cell activation-decreases the severity of cerulein-induced acute pancreatitis. World J Gastroenterol 2006; 12(8): 1219-1224 [PMID: 16534874 DOI: 10.3748/wjg.v12.i8.1219]
