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World J Gastroenterol. Feb 7, 2006; 12(5): 780-783
Published online Feb 7, 2006. doi: 10.3748/wjg.v12.i5.780
Increased heat shock protein 70 expression in the pancreas of rats with endotoxic shock
Xue-Lian Wang, Ying Li, Jin-Song Kuang, Yue Zhao, Pei Liu
Xue-Lian Wang, Ying Li, Pei Liu, Department of Infectious Diseases, The Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Jin-Song Kuang, Department of Internal Medicine, the Fourth People’s Hospital of Shenyang, Shenyang 110007, Liaoning Province, China
Yue Zhao, Department of Infectious diseases, the First Affiliated Hospital of Jinzhou Medical College, Jinzhou 121004, Liaoning Province, China
Supported by the National Natural Science Foundation of China, No. 30170849
Correspondence to: Dr. Pei Liu, Department of Infectious Diseases, The Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. syliupei2003@yahoo.com.cn
Telephone: +86-24-83956451 Fax: +86-24-83956451
Received: February 24, 2005
Revised: July 9, 2005
Accepted: July 12, 2005
Published online: February 7, 2006
Abstract

AIM: To investigate the ultra-structural changes and heat shock protein 70 (HSP70) expression in the pancreas of rats with endotoxic shock and to detect their possible relationship.

METHODS: A total of 33 Wistar rats were randomly divided into three groups: control group (given normal saline), small dose lipopolysaccharide (LPS) group (given LPS 5 mg/kg) and large dose LPS group (given LPS 10 mg/kg). Pancreas was explanted to detect the ultra-structural changes by TEM and the HSP70 expression by immunohistochemistry and Western blot.

RESULTS: Rats given small doses of LPS showed swelling and loss of mitochondrial cristae of acinar cells and increased number of autophagic vacuoles in the cytoplasm of acinar cells. Rats given large doses of LPS showed swelling, vacuolization, and obvious myeloid changes of mitochondrial cristae of acinar cells, increased number of autophagic vacuoles in the cytoplasm of acinar cells. HSP70 expression was increased compared to the control group (P<0.05).

CONCLUSION: Small doses of LPS may induce stronger expression of HSP70, promote autophagocytosis and ameliorate ultra-structural injuries.

Keywords: HSP70; Pancreas; Lipopolysaccharide; Ultra-structural changes