Basic Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Nov 28, 2006; 12(44): 7143-7148
Published online Nov 28, 2006. doi: 10.3748/wjg.v12.i44.7143
Elenoside increases intestinal motility
E Navarro, SJ Alonso, R Navarro, J Trujillo, E Jorge
E Navarro, SJ Alonso, Department of Pharmacology, Faculty of Medicine, University of La Laguna, 38071 La Laguna, Apdo. 55, Tenerife, Canary Islands, Spain
R Navarro, Department of Medical and Chirurgical Sciences, University of Las Palmas, Gran Canaria, Canary Islands, Spain
J Trujillo, E Jorge, Centro de Productos Naturales y Agrobiologia, CSIC, Tenerife, Canary Islands, Spain
Correspondence to: E Navarro, Department of Pharmacology, Faculty of Medicine, University of La Laguna, 38071 La Laguna, Apdo. 55, Tenerife, Canary Islands, Spain. enavarro@ull.es
Telephone: +34-922319345
Received: May 11, 2006
Revised: May 28, 2006
Accepted: October 17, 2006
Published online: November 28, 2006
Abstract

AIM: To study the effects of elenoside, an arylnaph-thalene lignan from Justicia hyssopifolia, on gastro-intestinal motility in vivo and in vitro in rats.

METHODS: Routine in vivo experimental assessments were catharsis index, water percentage of boluses, intestinal transit, and codeine antagonism. The groups included were vehicle control (propylene glycol-ethanol-plant oil-tween 80), elenoside (i.p. 25 and 50 mg/kg), cisapride (i.p. 10 mg/kg), and codeine phosphate (intragastric route, 50 mg/kg). In vitro approaches used isolated rat intestinal tissues (duodenum, jejunum, and ileum). The effects of elenoside at concentrations of 3.2 x 10-4, 6.4 x 10-4 and 1.2 x 10-3 mol/L, and cisapride at 10-6 mol/L were investigated.

RESULTS: Elenoside in vivo produced an increase in the catharsis index and water percentage of boluses and in the percentage of distance traveled by a suspension of activated charcoal. Codeine phosphate antagonized the effect of 25 mg/kg of elenoside. In vitro, elenoside in duodenum, jejunum and ileum produced an initial decrease in the contraction force followed by an increase. Elenoside resulted in decreased intestinal frequency in duodenum, jejunum, and ileum. The in vitro and in vivo effects of elenoside were similar to those produced by cisapride.

CONCLUSION: Elenoside is a lignan with an action similar to that of purgative and prokinetics drugs. Elenoside, could be an alternative to cisapride in treatment of gastrointestinal diseases as well as a preventive therapy for the undesirable gastrointestinal effects produced by opioids used for mild to moderate pain.

Keywords: Elenoside; Gastrointestinal motility; Small intestine preparation