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World J Gastroenterol. Nov 21, 2006; 12(43): 7012-7018
Published online Nov 21, 2006. doi: 10.3748/wjg.v12.i43.7012
Steroids reduce local inflammatory mediator secretion and mucosal permeability in collagenous colitis patients
Yesuf Taha, Yngve Raab, Marie Carlson, Anders Larsson, Mikael Lördal, Lars Lööf, Magnus Thörn
Yesuf Taha, Departments of Medical Sciences, University Hospital, Uppsala, Sweden
Yngve Raab, Magnus Thörn, Departments of Surgery, University Hospital, Uppsala, Sweden
Marie Carlson, Anders Larsson, Departments of Clinical Chemistry, University Hospital, Uppsala, Sweden
Yesuf Taha, Mikael Lördal, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Sweden
Lars Lööf, Center for Clinical Research, Central Hospital, Västerås, Sweden
Magnus Thörn, Department of Surgery, Karolinska Institutet, South Stockholm General Hospital, Stockholm, Sweden
Author contributions: All authors contributed equally to the work.
Supported by grants from the Swedish Medical Research Council, the Swedish Society of Medicine, the Medical Faculty of Uppsala University and Department of Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge
Correspondence to: Yesuf Taha, MD, Department of Gastroenterology, Karolinska Institutet, Karolinska University Hospital Hu-ddinge, S-141 86 Stockholm, Sweden. yesuf.taha@medsci.uu.se
Telephone: +46-8-58580000 Fax: +46-8-58582335
Received: September 7, 2006
Revised: October 2, 2006
Accepted: October 9, 2006
Published online: November 21, 2006
Abstract

AIM: To study the effect of oral steroids upon clinical response and rectal mucosa secretion of eosinophil cationic protein (ECP), myeloperoxidase (MPO), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and albumin in patients with collagenous colitis (CC).

METHODS: A segmental perfusion technique was used to collect perfusates from rectum of CC patients once before and twice (one and four weeks) after the start of steroid treatment. Clinical data was monitored and ECP, MPO, bFGF, VEGF and albumin concentrations were analyzed by immunochemical methods in perfusates and in serum.

RESULTS: Steroids reduced the number of bowel movements by more than five times within one week and all patients reported improved subjective well-being at wk 1 and 4. At the same time, the median concentrations of ECP, bFGF, VEGF and albumin in rectal perfusates decreased significantly. MPO values were above the detection limit in only 3 patients before treatment and in none during treatment. VEGF, bFGF, ECP and albumin concentrations correlated with each other with the exception of ECP and albumin. A decrease of serum ECP and VEGF concentrations was also seen even if the overtime reduction was not significant.

CONCLUSION: Oral steroid treatment in CC patients induced a simultaneous reduction of bowel movements and rectal release of ECP, bFGF, VEGF and albumin, suggesting that these polypeptides and increased mucosal permeability are important components of the pathophysiology in collagenous colitis.

Keywords: Collagenous colitis; Inflammatory mediator; Steroid treatment; Eosinophil cationic protein; Basic fibroblast growth factor; Vascular endothelial growth factor