Expression of 5-Lipoxygenase in human colorectal cancer

doi:10.3748/wjg.v12.i39.6355

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Oct 21, 2006 (publication date) through Nov 5, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 21, 2006; 12(39): 6355-6360
Published online Oct 21, 2006. doi: 10.3748/wjg.v12.i39.6355

Expression of 5-Lipoxygenase in human colorectal cancer

Labile Togba Soumaoro, Satoru Iida, Hiroyuki Uetake, Megumi Ishiguro, Yoko Takagi, Tetsuro Higuchi, Masamichi Yasuno, Masayuki Enomoto, Kenichi Sugihara

Labile Togba Soumaoro, Satoru Iida, Megumi Ishiguro, Yoko Takagi, Tetsuro Higuchi, Masamichi Yasuno, Masayuki Enomoto, Kenichi Sugihara, Department of Surgical Oncology, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8519, Japan

Hiroyuki Uetake, Department of Translational Oncology; Graduate School, Tokyo Medical and Dental University, Tokyo 113-8519, Japan

Correspondence to: Labile Togba Soumaoro, Service de Chirurgie generale, C.H.U. Ignace Deen, BP: 6103 Conakry, Guinea. soumaorol@yahoo.com

Telephone: +224-414336 Fax: +224-414336

Received: April 20, 2006
Revised: April 28, 2006
Accepted: May 22, 2006
Published online: October 21, 2006

Abstract

AIM: To evaluate the 5-lipoxygenases (Loxs) expression level in human colorectal cancer specimens in order to determine its clinicopathologic significance in human tumorigenesis.

METHODS: The relative quantity of 5-Lox mRNA in paired 91 colorectal tumor and adjacent normal mucosa samples was determined by real time quantitative PCR. Additionally, the expression of 5-Lox and cyclooxygenase (Cox)-2 proteins was also examined using immunohistochemical staining methods.

RESULTS: There was a marked increase in 5-Lox mRNA levels in the tumor compared with paired normal mucosa samples (P < 0.0001). Sixty six (72.5%) tumors showed high 5-Lox mRNA levels. The positivity rate of 5-Lox and Cox-2 protein expression was 68.7% and 79.1% respectively. There was a significant association between tumoral 5-Lox mRNA level and tumor size (Rho = 0.392, P = 0.0002), depth or vessel invasion.

CONCLUSION: These results suggest that 5-Lox is up-regulated in colorectal cancer and that inhibition of its expression might be valuable in the prevention and treatment of colorectal cancer.

Keywords: Arachidonic acid; 5-Lipoxygenase; Cyclooxygenase-2; Real time PCR; Immunohisto-chemistry; Colorectal cancer