Basic Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Sep 28, 2006; 12(36): 5805-5812
Published online Sep 28, 2006. doi: 10.3748/wjg.v12.i36.5805
Effect of intestinal lymphatic circulation blockage in two-hit rats
Chun-Yu Niu, Ji-Cheng Li, Zi-Gang Zhao, Jing Zhang, Xue-Hui Shao
Chun-Yu Niu, Ji-Cheng Li, Institute of Cell Biology, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Chun-Yu Niu, Zi-Gang Zhao, Jing Zhang, Xue-Hui Shao, Department of Pathophysiololgy, Hebei North University, Zhangjiakou 075029, Hebei Province, China
Author contributions: All authors contributed equally to the work.
Supported by National Science Foundation of China, No. 30370561; Natural Science Foundation of Hebei Province, No. C2004000649
Correspondence to: Professor Ji-Cheng Li, Institute of Cell Biology, Zhejiang University, Hangzhou 310003, Zhejiang Province, China. lijichen@zju.edu.cn
Telephone: +86-313-8041616 Fax: +86-313-8041616
Received: December 14, 2005
Revised: May 15, 2006
Accepted: May 24, 2006
Published online: September 28, 2006
Abstract

AIM: To study the effect of blocking intestinal lymphatic circulation in two-hit rats and explore the significance of intestinal lymphatic circulation in two-hit.

METHODS: Wistar rats were divided equally into three groups: mesenteric lymph duct ligation group, non-ligation group and sham group. Mesenteric lymph was diverted by ligation of mesenteric lymph duct, and the two-hit model was established by hemorrhage and lipopolysaccharide (LPS) methods. All rats were sampled for serum pre-experiment and 24 h post-experiment. The organs including kidney, liver, lung and heart were collected for pathomorphologic observation and biochemical investigation. The nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined in serum and tissue homogenate.

RESULTS: Pathomorphology study showed that the structures of kidney, lung, liver and heart tissues were normal in sham group; congestion, degeneration and necrosis in non-ligation group; but only mild lesions in ligation group. After two-hits, the contents of AST, ALT, BUN, Cr and LDH-1 in the serum of non-ligation group and ligation group were obviously higher than that in pre-experiment group and sham group, but obviously lower than that in non-ligation group. The contents of NO2-/NO3-, NOS, iNOS and MDA in the serum of non-ligation group were significantly increased, compared with pre-experiment and sham group, but SOD was significantly lower. These parameters were significantly different in ligation group compared with that in sham group, but NO2-/NO3-, iNOS and MDA in ligation group were significantly lower than that in non-ligation group.

CONCLUSION: Ligation of mesenteric lymph duct could improve the disturbance of organic function and morphologic damage in two-hit rats; the lymphatic mechanism in two-hit should be emphasized.

Keywords: Two-hit; Mesenteric lymph duct; Ligation; Organs; Humoral factor