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Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jul 7, 2006; 12(25): 4056-4060
Published online Jul 7, 2006. doi: 10.3748/wjg.v12.i25.4056
Expression of T-STAR gene is associated with regulation of telomerase activity in human colon cancer cell line HCT-116
Ling Zhang, Lian Guo, Yong Peng, Bing Chen
Ling Zhang, Department of Disease Prevention and Health Protection, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China
Lian Guo, Yong Peng, Bing Chen, Department of Endo-crinology, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30271442, No. 39980010
Correspondence to: Dr. Bing Chen, Department of Endocrinology, Southwest Hospital, 30 Gaotanyan, Sapingba, Chongqing 400038, China. chenb@mail.tmmu.com.cn
Telephone: +86-23-68754138
Received: February 8, 2006
Revised: March 17, 2006
Accepted: March 27, 2006
Published online: July 7, 2006
Abstract

AIM: To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or partial antisense cDNA into human colon cancer cell line HCT-116.

METHODS: mRNA and protein expression levels of T-STAR gene were determined by RT-PCR and western blot, and telomerase activity was measured by PCR-ELISA, after transfection of T-STAR sense or antisense gene into HCT-116 cells with lipofectamine.

RESULTS: T-STAR gene expression was enhanced or knocked down both at mRNA and protein levels, and telomerase activity was significantly increased or decreased.

CONCLUSION: The T-STAR gene may participate in regulation of telomerase activity in human colon cancer HCT-116 cells in a parallel fashion.

Keywords: T-STAR; Telomerase; Human colon cancer cells; Cell transfection