Itoh K, Sawasaki Y, Takeuchi K, Kato S, Imai N, Kato Y, Shibata N, Kobayashi M, Moriguchi Y, Higuchi M, Ishihata F, Sudoh Y, Miura S. Erythropoietin -induced proliferation of gastric mucosal cells. World J Gastroenterol 2006; 12(2): 234-239 [PMID: 16482623 DOI: 10.3748/wjg.v12.i2.234]
Corresponding Author of This Article
Kazuro Itoh, MD, PhD, Second Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa City, Saitama 359-8513, Japan. kazuito@ndmc.ac.jp,
Article-Type of This Article
Basic Research
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Yushi Sudoh, Kisen Hospital, Katsushika-ku, Tokyo, Japan
Supported by the grants from National Defense Medical College.
Correspondence to: Kazuro Itoh, MD, PhD, Second Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa City, Saitama 359-8513, Japan. kazuito@ndmc.ac.jp,
Telephone: +8-4-995-609 Fax: +8-4-2996-5201
Received: June 19, 2005 Revised: June 28, 2005 Accepted: July 15, 2005 Published online: January 14, 2006
Abstract
AIM: To analyze the localization of erythropoietin receptor on gastric specimens and characterize the effects of erythropoietin on the normal gastric epithelial proliferation using a porcine gastric epithelial cell culture model.
METHODS: Erythropoietin receptor was detected by RT-PCR, Western blotting and immunohistochermistry. Growth stimulation effects of erythropoietin on cultured gastric mucosal cells were determined by ELISA using bromodeoxyuridine (BrdU).
RESULTS: Erythropoietin receptor was detected on cultured porcine gastric mucosal epithelial cells. Erythropoietin receptor was also detected histochemically at the base of gastric mucosal epithelium. BrdU assay demonstrated a dose-dependent increase in growth potential of cultured porcine gastric mucosal epithelial cells by administration of erythropoietin, as well as these effects were inhibited by administration of anti- erythropoietin antibody (P < 0.01).
CONCLUSION: These findings indicate that erythropoietin has a potential to proliferate gastric mucosal epithelium via erythropoietin receptor.
