Itoh K, Sawasaki Y, Takeuchi K, Kato S, Imai N, Kato Y, Shibata N, Kobayashi M, Moriguchi Y, Higuchi M, Ishihata F, Sudoh Y, Miura S. Erythropoietin -induced proliferation of gastric mucosal cells. World J Gastroenterol 2006; 12(2): 234-239 [PMID: 16482623 DOI: 10.3748/wjg.v12.i2.234]
Corresponding Author of This Article
Kazuro Itoh, MD, PhD, Second Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa City, Saitama 359-8513, Japan. kazuito@ndmc.ac.jp,
Article-Type of This Article
Basic Research
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Yushi Sudoh, Kisen Hospital, Katsushika-ku, Tokyo, Japan
Supported by the grants from National Defense Medical College.
Correspondence to: Kazuro Itoh, MD, PhD, Second Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa City, Saitama 359-8513, Japan. kazuito@ndmc.ac.jp,
Telephone: +8-4-995-609 Fax: +8-4-2996-5201
Received: June 19, 2005 Revised: June 28, 2005 Accepted: July 15, 2005 Published online: January 14, 2006
Abstract
AIM: To analyze the localization of erythropoietin receptor on gastric specimens and characterize the effects of erythropoietin on the normal gastric epithelial proliferation using a porcine gastric epithelial cell culture model.
METHODS: Erythropoietin receptor was detected by RT-PCR, Western blotting and immunohistochermistry. Growth stimulation effects of erythropoietin on cultured gastric mucosal cells were determined by ELISA using bromodeoxyuridine (BrdU).
RESULTS: Erythropoietin receptor was detected on cultured porcine gastric mucosal epithelial cells. Erythropoietin receptor was also detected histochemically at the base of gastric mucosal epithelium. BrdU assay demonstrated a dose-dependent increase in growth potential of cultured porcine gastric mucosal epithelial cells by administration of erythropoietin, as well as these effects were inhibited by administration of anti- erythropoietin antibody (P < 0.01).
CONCLUSION: These findings indicate that erythropoietin has a potential to proliferate gastric mucosal epithelium via erythropoietin receptor.
Itoh K, Sawasaki Y, Takeuchi K, Kato S, Imai N, Kato Y, Shibata N, Kobayashi M, Moriguchi Y, Higuchi M, Ishihata F, Sudoh Y, Miura S. Erythropoietin -induced proliferation of gastric mucosal cells. World J Gastroenterol 2006; 12(2): 234-239 [PMID: 16482623 DOI: 10.3748/wjg.v12.i2.234]
