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World J Gastroenterol. May 14, 2006; 12(18): 2936-2940
Published online May 14, 2006. doi: 10.3748/wjg.v12.i18.2936
Effects of ischemic preconditioning on cyclinD1 expression during early ischemic reperfusion in rats
Fang-Gang Cai, Jian-Sheng Xiao, Qi-Fa Ye
Fang-Gang Cai, Department of General Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, Fujian Province, China
Jian-Sheng Xiao, Department of General Surgery, First Affiliated Hospital, Jiangxi Medical College, Nanchang 330000, Jiangxi Province, China
Qi-Fa Ye, Xiangya Medical Transplantation Academy of Central South University, Changsha 410013, Hunan Province, China
Supported by Youth Foundation of Health Bureau of Fujian Province, No. 2003-1-19
Correspondence to: Dr. Fang-Gang Cai, Department of General Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, Fujian Province, China. cfg453@163.com
Telephone: +86-951-87982550
Received: September 9, 2005
Revised: September 28, 2005
Accepted: October 26, 2005
Published online: May 14, 2006
Abstract

AIM: To observe the effect of ischemic preconditioning on cyclinD1 expression in rat liver cells during early ischemic reperfusion.

METHODS: Fifty-four SD rats were randomly divided into ischemic preconditioning group (IP), ischemia/reperfusion group (IR) and sham operation group (SO). The IP and IR groups were further divided into four sub-groups (n = 6). Sham operation group (SO) served as the control group (n = 6). A model of partial liver ischemia/reperfusion was used, in which rats were subjected to liver ischemia for 60 min prior to reperfusion. The animals in the IP group underwent ischemic preconditioning twice for 5 min each time prior to the ischemia/reperfusion challenge. After 0, 1, 2, and 4 h of reperfusion, serum and liver tissue in each group were collected to detect the level of serum ALT, liver histopathology and expression of cyclinD1 mRNA and protein. Flow cytometry was used to detect cell cycle as the quantity indicator of cell regeneration.

RESULTS: Compared with IR group, IP group showed a significantly lower ALT level in 1 h to 4 h sub-groups (P < 0.05). Proliferation index(PI) indicated by the S-phase and G2/M-phase ratio [(S+G2/M)/(G0/G1+S+G2/M)] was significantly increased in IP group at 0 and 1 h (26.44± 7.60% vs 18.56 ± 6.40%,41.87 ± 7.27% vs 20.25 ± 6.70%, P < 0.05). Meanwhile, cyclinD1 protein expression could be detected in IP group. But in IR group, cyclinD1 protein expression occurred 2 h after reperfusion. The expression of cyclinD1 mRNA increased significantly in IP group at 0 and 1 h (0.568 ± 0.112 vs 0.274 ± 0.069, 0.762 ± 0.164 vs 0.348 ± 0.093, P < 0.05).

CONCLUSION: Ischemic preconditioning can protect liver cells against ischemia/reperfusion injury, which may be related to cell proliferation and expression of cyclinD1 during early ischemic reperfusion.

Keywords: Liver; Ischemic preconditioning; CyclinD1; Proliferation