Basic Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. May 14, 2006; 12(18): 2908-2913
Published online May 14, 2006. doi: 10.3748/wjg.v12.i18.2908
Hyperlipidemia intensifies cerulein-induced acute pancreatitis associated with activation of protein kinase C in rats
Ya-Jun Wang, Jia-Bang Sun, Fei Li, Shu-Wen Zhang
Ya-Jun Wang, Jia-Bang Sun, Fei Li, Shu-Wen Zhang, Department of General Surgery, Xuanwu Hospital of Capital University of Medical Sciences, Beijing 100053, China
Supported by the Key Program of Beijing Academy of Health, No.1998-11-2001-09
Correspondence to: Dr. Ya-Jun Wang, Department of General Surgery, Xuanwu Hospital of Capital University of Medical Sciences, 45 Changchun Street, Beijing 100053, China. wyjwmj@yahoo.com.cn
Telephone: +86-10-81439530
Received: September 11, 2005
Revised: September 28, 2005
Accepted: November 18, 2005
Published online: May 14, 2006
Abstract

AIM: To investigate the effects of hyperlipidemia on acute pancreatitis (AP) and the possible mechanisms.

METHODS: Rat models of hyperlipidemia and AP were established by Triton WR1339 and cerulein respectively. Human albumin was used to treat AP complicated by hyperlipidemia. In each group, we compared the histological score, volume of ascites, ratio of pancreatic wet/dry weight, serum amylase (AMY) and pancreatic acinar cell apoptosis. The level of protein kinase C (PKC) membrane translocation in pancreatic tissue was detected by Western blot.

RESULTS: In the hyperlipidemia model established by Triton WR1339, triglyceride (TG) increased remarkably and reached its peak 6 h after injection, and most rats developed mild acute pancreatitis. Histological score, volume of ascites, ratio of wet/dry weight and serum AMY in AP animals with hyperlipidemia were obviously higher than those in AP animals (P < 0.05) and decreased after albumin therapy but not significantly (P > 0.05). Apoptotic cells detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) increased in AP animals with hyperlipidemia and did not change distinctly after albumin therapy. PKC membrane translocation level increased in AP animals with hyperlipidemia and decreased remarkably after albumin therapy (P < 0.05).

CONCLUSION: Hyperlipidemia may induce AP or intensify pancreatic injury. Albumin therapy can not alleviate pancreatic lesion effectively. PKC activation may be one mechanism by which AP is intensified by hyperlipidemia.

Keywords: Acute pancreatitis; Hyperlipidemia; Protein kinase C