Liu Y, Wang LF, Zou HF, Song XY, Xu HF, Lin P, Zheng HH, Yu XG. Expression and location of Smad2, 4 mRNAs during and after liver fibrogenesis of rats. World J Gastroenterol 2006; 12(10): 1577-1582 [PMID: 16570350 DOI: 10.3748/wjg.v12.i10.1577]
Corresponding Author of This Article
Dr. Xiao-Guang Yu, Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin 150086, Heilongjiang Province, China. coollya@gmail.com
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Yang Liu, Hai-Feng Zou, Hua-Feng Xu, Ping Lin, Xiao-Guang Yu, Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Li-Feng Wang, Department of Pathology of the First Clinical College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Xiao-Yan Song, Master degree student of Department of Pathology of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Hai-Hong Zheng, Department of Experimental Animal of the Second Clinical College, Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Research Foundation of Department of Education of Heilongjiang Province, No.10551131
Correspondence to: Dr. Xiao-Guang Yu, Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin 150086, Heilongjiang Province, China. coollya@gmail.com
Telephone: +86-451-86671684
Received: March 22, 2005 Revised: April 21, 2005 Accepted: July 21, 2005 Published online: March 14, 2006
Abstract
AIM: To investigate the location alteration of Smad2 and Smad4 mRNAs in the liver during and after fibrogenesis in rats.
METHODS: Eighty male Wistar rats weighing approximately 200 g each were used. The rat models of experimental hepatic fibrosis were established by injection with carbon tetrachloride (CCl4), normal rats and rats were injected with olive oil and served as control groups. In situ hybridization(ISH) was used to detect the Smad2 and Smad4 mRNA in liver.
RESULTS: In situ hybridization showed Smad2 and Smad4 mRNA expressions in the cytoplasm of hepatic stellate cells (HSC), fibroblasts and myofibroblasts around the central vein and hepatic sinus during and after fibrogenesis. Expression of Smad2, 4 mRNA was higher than that in normal and control rats.
CONCLUSION: In the process of and after hepatic fibrosis formation, HSC, fibroblasts and myofibroblasts are the major cells that express Smad2 and Smad4. The more serious the hepatic fibrosis is in the injured liver, the higher the level of Smad2 and Smad4 gene expression is during and after fibrogenesis respectively.
