Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 14, 2005; 11(6): 850-853
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.850
Cysteine peptidase and its inhibitor activity levels and vitamin E concentration in normal human serum and colorectal carcinomas
Robert Szwed, Zygmunt Grzebieniak, Yousif Saleh, Godwin Bwire Ekonjo, Maciej Siewinski
Robert Szwed, Zygmunt Grzebieniak, Clinic of Surgery and Oncology, Wroclaw Medical University, 2 Sklodowskiej-curie Street, 50-368 Wroclaw, Poland
Yousif Saleh, Godwin Bwire Ekonjo, Department of Obstetrics and Gynaecology, Wroclaw Medical University, 3 Chalubenskiego Street, 50-368 Wroclaw, Poland
Maciej Siewinski, Faculty of Public Health, Medical University of Wroclaw; Bartla Street, 5, 50 -618 Wroclaw, Poland
Author contributions: All authors contributed equally to the work.
Correspondence to: Maciej Siewinski, Ph.D., Faculty of Public Health, Medical University of Wroclaw; Bartla Street, 5, 50-618 Wroclaw, Poland. siewinski@op.pl
Telephone: +48-71-7842413 Fax: +48-71-7840111
Received: April 15, 2004
Revised: April 17, 2004
Accepted: June 7, 2004
Published online: February 14, 2005
Abstract

AIM: Cysteine peptidase (CP) and its inhibitor (CPI) are a matrix protease that may be associated with colorectal carcinoma invasion and progression, and vitamin E is also a stimulator of the immunological system. Our purpose was to determine the correlation between the expression of cysteine peptidases and their endogenous inhibitors, and the level of vitamin E in sera of patients with colorectal cancer in comparison with healthy individuals.

METHODS: The levels of cysteine peptidases and their inhibitors were determined in the sera of patients with primary and metastatic colorectal carcinoma and healthy individuals using fluorogenic substrate, and the level of vitamin E was determined by HPLC.

RESULTS: The levels of cysteine peptidases and their inhibitors were significantly higher in the metastatic colorectal cancer patients than that in the healthy controls (P<0.05). The activity of CP increased 2.2-fold, CPI 2.8-fold and vitamin E decreased 3.4-fold in sera of patients with metastasis in comparison with controls. The level of vitamin E in healthy individuals was higher, whereas the activity of cysteine peptidases and their inhibitors associated with complexes was lower than that in patients with cancer of the digestive tract.

CONCLUSION: These results suggest that the serum levels of CP and their inhibitors could be an indicator of the prognosis for patients with metastatic colorectal cancer. Vitamin E can be administered prophylactically to prevent digestive tract neoplasmas.

Keywords: Cysteine peptidases; Inhibitors; Vitamin E; Colorectal cancers