Determination of platelet-activating factor by reverse phase high-performance liquid chromatography and its application in viral hepatitis

doi:10.3748/wjg.v11.i46.7364

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 14, 2005; 11(46): 7364-7367
Published online Dec 14, 2005. doi: 10.3748/wjg.v11.i46.7364

Determination of platelet-activating factor by reverse phase high-performance liquid chromatography and its application in viral hepatitis

Hong-Cui Cao, Xiao-Ming Chen, Wei Xu

Hong-Cui Cao, Xiao-Ming Chen, Wei Xu, Department of Infectious Diseases, First Affiliated Hospital, Medical School of Zhejiang University; Key Laboratory of Infectious Diseases of Ministry of Public Health, Hangzhou 310003, Zhejiang Province, China

Author contributions: All authors contributed equally to the work.

Supported by a Health Foundation of Zhejiang Province, No. 2004C083, 2004B068

Correspondence to: Xiao-Ming Chen, Department of Infectious Disease, First Affiliated Hospital, Medical School of Zhejiang University; Key Laboratory of Infectious Disease of Ministry of Public Health, Hangzhou 310003, Zhejiang Province, China. shi9876@hzcnc.com

Telephone: +86-571-87236759

Received: June 21, 2005
Revised: July 21, 2005
Accepted: July 21, 2005
Published online: December 14, 2005

Abstract

AIM: To detect the platelet-activating factor (PAF) and the plasma or serum levels of tumor necrosis factor-α (TNF-α) malondialdehyde (MDA), endotoxin (ET) and to discuss their significance in various types of viral hepatitis.

METHODS: PAF, TNF-α, MDA, and ET levels in 60 controls, 16 cases of acute viral hepatitis, 71 cases of chronic viral hepatitis, 19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography (rHPLC), bio-assay, ELISA, thiobarbituric acid (TBA), and limulus lysate test (LLT), respectively.

RESULTS: The rHPLC was more sensitive and specific than bio-assay (r = 0.912, P<0.01). The plasma levels of PAF, TNF-α, MDA, and ET in patients with viral hepatitis were higher than those in controls (P<0.01).

CONCLUSION: rHPLC is more reliable and accurate for the detection of PAF.

Keywords: Platelet-activating factor; Malondialdehyde; Endotoxin; rHPLC; Viral hepatitis