Determination of platelet-activating factor by reverse phase high-performance liquid chromatography and its application in viral hepatitis

doi:10.3748/wjg.v11.i46.7364

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 11, Issue 46

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (2654)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-2) series.

Item

Count

PDF

259

HTML

2032

Figures (1-2)

363

Sum=2654

Dec 14, 2005 (publication date) through Nov 27, 2024

Times Cited of This Article

Times Cited (4)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Rapid Communication

World J Gastroenterol. Dec 14, 2005; 11(46): 7364-7367
Published online Dec 14, 2005. doi: 10.3748/wjg.v11.i46.7364

Determination of platelet-activating factor by reverse phase high-performance liquid chromatography and its application in viral hepatitis

Hong-Cui Cao, Xiao-Ming Chen, Wei Xu

Hong-Cui Cao, Xiao-Ming Chen, Wei Xu, Department of Infectious Diseases, First Affiliated Hospital, Medical School of Zhejiang University; Key Laboratory of Infectious Diseases of Ministry of Public Health, Hangzhou 310003, Zhejiang Province, China

Author contributions: All authors contributed equally to the work.

Supported by a Health Foundation of Zhejiang Province, No. 2004C083, 2004B068

Correspondence to: Xiao-Ming Chen, Department of Infectious Disease, First Affiliated Hospital, Medical School of Zhejiang University; Key Laboratory of Infectious Disease of Ministry of Public Health, Hangzhou 310003, Zhejiang Province, China. shi9876@hzcnc.com

Telephone: +86-571-87236759

Received: June 21, 2005
Revised: July 21, 2005
Accepted: July 21, 2005
Published online: December 14, 2005

Abstract

AIM: To detect the platelet-activating factor (PAF) and the plasma or serum levels of tumor necrosis factor-α (TNF-α) malondialdehyde (MDA), endotoxin (ET) and to discuss their significance in various types of viral hepatitis.

METHODS: PAF, TNF-α, MDA, and ET levels in 60 controls, 16 cases of acute viral hepatitis, 71 cases of chronic viral hepatitis, 19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography (rHPLC), bio-assay, ELISA, thiobarbituric acid (TBA), and limulus lysate test (LLT), respectively.

RESULTS: The rHPLC was more sensitive and specific than bio-assay (r = 0.912, P<0.01). The plasma levels of PAF, TNF-α, MDA, and ET in patients with viral hepatitis were higher than those in controls (P<0.01).

CONCLUSION: rHPLC is more reliable and accurate for the detection of PAF.

Keywords: Platelet-activating factor; Malondialdehyde; Endotoxin; rHPLC; Viral hepatitis