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World J Gastroenterol. Nov 28, 2005; 11(44): 7033-7035
Published online Nov 28, 2005. doi: 10.3748/wjg.v11.i44.7033
Cell cycle and radiosensitivity of progeny of irradiated primary cultured human hepatocarcinoma cells
Zhi-Zhong Liu, Wen-Ying Huang, Ju-Sheng Lin, Xiao-Sheng Li, Kuo-Huan Liang, Jia-Long Huang
Zhi-Zhong Liu, Ju-Sheng Lin, Kuo-Huan Liang, Jia-Long Huang, Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Wen-Ying Huang, The People’s Hospital of Chenzhou City, Chenzhou 423000, Hunan Province, China
Xiao-Sheng Li, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Zhi-Zhong Liu, Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. whzzl@163.net
Telephone: +86-27-13554460933
Received: June 18, 2004
Revised: August 16, 2004
Accepted: August 20, 2004
Published online: November 28, 2005
Abstract

AIM: To evaluate the change of growth characteristics and radiosensitivity of irradiated primary cultured human hepatocarcinoma cells.

METHODS: All tumor tissue samples were obtained from 39 hepatocarcinoma patients with a mean age of 49.6 years (range 22-76 years). We divided the samples into irradiated group and non-irradiated group and measured their plating efficiency (PE), population doubling time (PDT), radiosensitivity index SF2 and cell cycle.

RESULTS: The PDT of primary culture of hepatocarcinoma cells was 91.0±6.6 h, PE was 12.0±1.4%, SF2 was 0.41±0.05%. The PDT of their irradiated progeny was 124.8±5.8 h, PE was 5.0±0.7%, SF2 was 0.65±0.09%. The primary cultured human hepatocarcinoma cells showed significant S reduction and G2 arrest in a dose-dependent manner. The progeny of irradiated primary cultured hepatocarcinoma cells grew more slowly and its radiosensitivity increased.

CONCLUSION: The progeny of irradiated primary cultured human hepatocarcinoma cells grows more slowly and its radiosensitivity increases.

Keywords: Hepatocarcinoma; Cell cycle; Population doubling time; Radiosensitivity