Oral vaccination of mice against rodent malaria with recombinant Lactococcus lactis expressing MSP-119

doi:10.3748/wjg.v11.i44.6975

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 11, Issue 44

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (2984)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-4) series.

Item

Count

PDF

320

HTML

2423

Figures (1-4)

241

Sum=2984

Nov 28, 2005 (publication date) through Nov 25, 2024

Times Cited of This Article

Times Cited (26)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Research

World J Gastroenterol. Nov 28, 2005; 11(44): 6975-6980
Published online Nov 28, 2005. doi: 10.3748/wjg.v11.i44.6975

Oral vaccination of mice against rodent malaria with recombinant Lactococcus lactis expressing MSP-1₁₉

Zhi-Hong Zhang, Pei-Hong Jiang, Ning-Jun Li, Mi Shi, Weida Huang

Zhi-Hong Zhang, Pei-Hong Jiang, Ning-Jun Li, Mi Shi, Weida Huang, Department of Biochemistry, School of Life Science, Fudan University, Shanghai 200433, China

Author contributions: All authors contributed equally to the work.

Supported by the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), No. 980198

Correspondence to: Weida Huang, Department of Biochemistry, Fudan University, 220 Han Dan Road, Shanghai 200433, China. whuang@fudan.edu.cn

Telephone: +86-21-65643446 Fax: +86-21-55522773

Received: September 18, 2004
Revised: November 15, 2004
Accepted: November 19, 2004
Published online: November 28, 2005

Abstract

AIM: To construct the recombinant Lactococcus lactis as oral delivery vaccination against malaria.

METHODS: The C-terminal 19-ku fragments of MSP1 (MSP-1₁₉) of Plasmodium yoelii 265-BY was expressed in L. lactis and the recombinant L. lactis was administered orally to BALB/c and C57BL/6 mice. After seven interval vaccinations within 4 wk, the mice were challenged with P. yoelii 265-BY parasites of erythrocytic stage. The protective efficacy of recombinant L. lactis was evaluated.

RESULTS: The peak parasitemias in average for the experiment groups of BALB/c and C57BL/6 mice were 0.8±0.4% and 20.8±26.5%, respectively, and those of their control groups were 12.0±0.8% and 60.8±9.6%, respectively. None of the BALB/c mice in both experimental group and control group died during the experiment. However, all the C57BL/6 mice in the control group died within 23 d and all the vaccinated mice survived well.

CONCLUSION: The results imply the potential of recombinant L. lactis as oral delivery vaccination against malaria.

Keywords: Lactococcus lactis; Oral delivery vaccination; Malaria