Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 21, 2005; 11(35): 5561-5564
Published online Sep 21, 2005. doi: 10.3748/wjg.v11.i35.5561
Heat shock protein 70 chaperoned alpha-fetoprotein in human hepatocellular carcinoma cell line BEL-7402
Xiao-Ping Wang, Qiao-Xia Wang, Hai-Yan Li, Rui-Fen Chen
Xiao-Ping Wang, Hai-Yan Li, Rui-Fen Chen, Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China
Qiao-Xia Wang, Department of Gastroenterology, the Third Clinical Hospital, Lanzhou Medical College, Lanzhou 730000, Gansu Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Research Fund for Young Scholars of Beijing, No. 02120031 and Research Program of Beijing Education Committee, No. 0410025002
Correspondence to: Dr. Xiao-Ping Wang, Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China. wxpphd@yahoo.com.cn
Telephone: +86-10-63051455-11
Received: January 19, 2005
Revised: February 15, 2005
Accepted: February 18, 2005
Published online: September 21, 2005
Abstract

AIM: To investigate the interaction between heat shock protein 70 (HSP70) and α-fetoprotein (AFP) in human hepatocellular carcinoma (HCC) cell line BEL-7402.

METHODS: The expression and localization of HSP70 and AFP in human HCC cell line BEL-7402 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. The interaction between HSP70 and AFP in HCC cells was analyzed by immunoprecipitation and Western blot.

RESULTS: Immunocytochemical staining detection showed that HCC cell BEL-7402 expressed a high level of HSP70 and AFP synchronously. Both were stained in cell plasma. AFP existed in the immunoprecipitate of anti-HSP70 mAb, while there was HSP70 in the immunoprecipitate of anti-AFP mAb.

CONCLUSION: HSP70 chaperones AFP in human HCC cell BEL-7402. The interaction between HSP70 and AFP in human HCC cell can be a new route to study the pathogenesis and immunotherapy of HCC.

Keywords: Heat shock protein 70; Alpha-fetoprotein; Hepatocellular carcinoma