Basic Research
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 28, 2005; 11(32): 4947-4952
Published online Aug 28, 2005. doi: 10.3748/wjg.v11.i32.4947
Hepatocytes targeting of cationic liposomes modified with soybean sterylglucoside and polyethylene glycol
Xian-Rong Qi, Wen-Wei Yan, Jing Shi
Xian-Rong Qi, Wen-Wei Yan, Jing Shi, Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30371265
Correspondence to: Professor Xian-Rong Qi, Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China. qixr2001@yahoo.com.cn
Telephone: +86-10-82801584 Fax: +86-10-62015584
Received: November 15, 2004
Revised: February 15, 2005
Accepted: February 18, 2005
Published online: August 28, 2005
Abstract

AIM: In this study, a hepatocyte-specific targeting technology was developed by modifying cationic liposomes with soybean sterylglucoside (SG) and polyethylene glycol (PEG) (C/SG/PEG-liposomes).

METHODS: The liposomal transfection efficiencies in HepG2 2.2.15 cells were estimated with the use of fluorescein sodium (FS) as a model drug, by flow cytometry. The antisense activity of C/SG/PEG-liposomes entrapped antisense oligonucleotides (ODN) was determined as HBsAg and HBeAg in HepG2 2.2.15 cells by ELISA. The liposome uptake by liver and liver cells in mice was carried out after intravenous injection of 3H-labeled liposomes.

RESULTS: C/SG-liposomes entrapped FS were effectively transfected into HepG2 2.2.15 cells in vitro. C/SG/PEG-liposomes entrapped ODN, reduced the secretion of both HBsAg and HBeAg in HepG2 2.2.15 cells when compared to free ODN. After in vivo injection of 3H-labeled C/SG/PEG-liposomes, higher radiation accumulation was observed in the hepatocytes than non-parenchymal cells of the liver.

CONCLUSION: C/SG/PEG-liposomes mediated gene transfer to the liver is an effective gene-delivery method for hepatocytes-specific targeting, which appears to have a potential for gene therapy of HBV infections.

Keywords: Hepatocytes targeting; Cationic liposomes; Soybean sterylglucoside