Chen KS, Zhang L, Tang L, Zhang YH, Gao DL, Yan L, Zhang L. Expression of heparanase mRNA in anti-sense oligonucleotide-transfected human esophageal cancer EC9706 cells. World J Gastroenterol 2005; 11(31): 4916-4917 [PMID: 16097072 DOI: 10.3748/wjg.v11.i31.4916]
Corresponding Author of This Article
Kui-Sheng Chen, Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Key Laboratory of Henan Tumor Pathology, Zhengzhou 450052, Henan Province, China. chenksh2002@yahoo.com.cn
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Brief Reports
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World J Gastroenterol. Aug 21, 2005; 11(31): 4916-4917 Published online Aug 21, 2005. doi: 10.3748/wjg.v11.i31.4916
Expression of heparanase mRNA in anti-sense oligonucleotide-transfected human esophageal cancer EC9706 cells
Kui-Sheng Chen, Lan Zhang, Lin Tang, Yun-Han Zhang, Dong-Ling Gao, Liang Yan, Lei Zhang
Kui-Sheng Chen, Lan Zhang, Lin Tang, Yun-Han Zhang, Dong-Ling Gao, Liang Yan, Lei Zhang, Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Key Laboratory of Henan Tumor Pathology, Zhengzhou 450052, Henan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Natural Science Foundation of Henan Province, No. 0311043700 and the Foundation for Young Mainstay Teachers in Colleges and universities of Henan Province, No.100(2003) and the Building Foundation for 211 Key Fields during the 15th Five-year Plan Period of Ministry of Education, No. 2(2002)
Correspondence to: Kui-Sheng Chen, Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Key Laboratory of Henan Tumor Pathology, Zhengzhou 450052, Henan Province, China. chenksh2002@yahoo.com.cn
Telephone: +86-371-6912412 Fax: +86-371-6658175
Received: February 17, 2005 Revised: April 1, 2005 Accepted: April 2, 2005 Published online: August 21, 2005
Abstract
AIM: To investigate the effects of anti-sense oligonucleotides (ASODNs) on mRNA expression of heparanase in human esophageal cancer EC9706 cells.
METHODS: One non-sense oligonucleotide (N-ODN) and five ASODNs against different heparanase mRNA sites were transfected into EC9706 cells, then the expression of heparanase mRNA in EC9706 cells was studied by in situ hybridization.
RESULTS: The expression of heparanase mRNA could be inhibited by ASODNs.There was no significant difference among five ASODNs (P>0.05), but there was a significant difference between ASODNs and N-ODN or non-transfected group (ASODN1: 2.25±0.25, ASODN2: 2.21±0.23, ASODN3: 2.23±0.23, ASODN4: 2.25±0.24 vs N-ODN: 3.47±2.80 or non- transfected group: 3.51±2.93 respectively,P<0.05).
CONCLUSION: The expression of heparanase mRNA in EC9706 cells can be inhibited by ASODNs in vivo, and heparanase ASODNs can inhibit metastasis of esophageal squamous cell carcinoma or other tumors by inhibiting the expression of heparanase.
