Colorectal Cancer
Copyright ©2005 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jan 21, 2005; 11(3): 327-330
Published online Jan 21, 2005. doi: 10.3748/wjg.v11.i3.327
In vivo and in situ detection of colorectal cancer using Fourier transform infrared spectroscopy
Qing-Bo Li, Zhi Xu, Neng-Wei Zhang, Li Zhang, Fan Wang, Li-Min Yang, Jian-Sheng Wang, Su Zhou, Yuan-Fu Zhang, Xiao-Si Zhou, Jing-Sen Shi, Jin-Guang Wu
Qing-Bo Li, Li Zhang, Fan Wang, Li-Min Yang, Yuan-Fu Zhang, Jin-Guang Wu, The State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Zhi Xu, Neng-Wei Zhang, Su Zhou, Xiao-Si Zhou, Department of General Surgery, Third Hospital of Peking University, Beijing 100083, China
Jian-Sheng Wang, Jing-Sen Shi, Department of Oncological Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30371604 and State Key Project of China, No. 2002CCA01900
Correspondence to: Jin-Guang Wu, The State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. wjg@chem.pku.edu.cn
Telephone: +86-10-62757951 Fax: +86-10-62751708
Received: April 9, 2004
Revised: April 12, 2004
Accepted: May 31, 2004
Published online: January 21, 2005
Abstract

AIM: Real-time and rapid identification of the malignant tissue can be performed during or before surgical operation. Here we aimed to detect in vivo and in situ colorectal cancer by using Fourier transform infrared (FTIR) spectroscopy and fiber-optic technology.

METHODS: A total of five patients with large intestine cancer were detected in vivo and in situ. Of them, three cases of colon cancer and one case of cecum cancer were detected intraoperatively and in vivo by using a FTIR spectrometer during surgical operation, and one case of rectum cancer was explored non-invasively and in vivo before the surgical operation. Normal and malignant colorectal tissues were detected in vivo and in situ using FTIR spectroscopy on the basis of fundamental studies.

RESULTS: There were significant differences between FTIR spectra of normal and malignant colorectal tissues detected in vivo and in situ. Experimental results revealed that the spectral characteristics of normal and malignant tissues found in vivo and in situ were similar to those obtained from in vitro measurement in our previous fundamental research.

CONCLUSION: FTIR fiber-optic attenuated total reflectance (ATR) spectroscopy can identify in situ and in vivo colorectal cancer. FTIR spectroscopic method with fiber optics is a non-invasive, rapid, accurate and in vivo cancer detection technique in clinical diagnosis.

Keywords: Colorectal cancer; Fourier transform infrared spectroscopy