Helicobacter Pylori
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 28, 2005; 11(28): 4357-4362
Published online Jul 28, 2005. doi: 10.3748/wjg.v11.i28.4357
Expression of CD86 and increased infiltration of NK cells are associated with Helicobacter pylori-dependent state of early stage high-grade gastric MALT lymphoma
Sung-Hsin Kuo, Li-Tzong Chen, Chi-Long Chen, Shin-Lian Doong, Kun-Huei Yeh, Ming-Shiang Wu, Tsui-Lien Mao, Hui-Chen Hsu, Hsiu-Po Wang, Jaw-Town Lin, Ann-Lii Cheng
Sung-Hsin Kuo, Departments of Oncology, National Taiwan University Hospital, Taipei; Cancer Research Center, and Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan, China
Li-Tzong Chen, Division of Cancer Research, National Health Research Institutes, Taipei; Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taipei, Taiwan, China
Chi-Long Chen, Department of Pathology, Taipei Medical University, Taipei, Taiwan, China
Shin-Lian Doong, Graduate Institute of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan, China
Kun-Huei Yeh, Hui-Chen Hsu, Departments of Oncology, National Taiwan University Hospital, Taipei; Cancer Research Center, National Taiwan University College of Medicine, Taipei, Taiwan, China
Ming-Shiang Wu, Jaw-Town Lin, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan, China
Tsui-Lien Mao, Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan, China
Hsiu-Po Wang, Department of Emergency Medicine, National Taiwan University Hospital, Taipei, Taiwan, China
Ann-Lii Cheng, Department of Internal Medicine and Department of Oncology, National Taiwan University Hospital, Taipei; Cancer Research Center, National Taiwan University College of Medicine, Taipei; Division of Cancer Research, National Health Research Institutes, Taipei, Taiwan, China
Author contributions: All authors contributed equally to the work.
Supported by the Research Grants, No. NSC91-3112-B-002-009, No. NSC92-3112-B-002-027, and No. NSC93-3112-B-002-007 from the National Science Council, No. NHRI-CN-CA9201S (92A084, and 93A059) from the National Health Research Institutes, and No. NTUH 93-N012, No. NTUH 94S155 from National Taiwan University Hospital, Taiwan, China
Correspondence to: Ann-Lii Cheng, MD, PhD, Department of Internal Medicine and Department of Oncology, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei, Taiwan, China. andrew@ha.mc.ntu.edu.tw
Telephone: +886-2-2312-3456-7251 Fax: +886-2-2371-1174
Received: November 4, 2004
Revised: December 3, 2004
Accepted: December 8, 2004
Published online: July 28, 2005
Abstract

AIM: A high percentage of early-stage high-grade gastric mucosa-associated lymphoid tissue (MALT) lymphomas remainHelicobacter pylori (H pylori)-dependent. However, unlike their low-grade counterparts, high-grade gastric MALT lymphomas may progress rapidly if unresponsive to H pylori eradication. It is mandatory to identify markers that may predict the H pylori-dependent status of these tumors. Proliferation of MALT lymphoma cells depends on cognate help and cell-to-cell contact of H pylori-specific intratumoral T-cells. To examine whether the expression of co-stimulatory marker CD86 (B7.2) and the infiltration of CD56 (+) natural killer (NK) cells can be useful markers to predict H pylori-dependent status of high-grade gastric MALT lymphoma.

METHODS: Lymphoma biopsies from 26 patients who had participated in a prospective study of H pylori-eradication for stage IE high-grade gastric MALT lymphomas were evaluated. Tumors that resolved to Wotherspoon grade II or less after H pylorieradication were classified as H pylori-dependent; others were classified as H pylori-independent. The infiltration of NK cells and the expression of CD86 in pre-treatment paraffin-embedded lymphoma tissues were determined by immunohistochemistry.

RESULTS: There were 16 H pylori-dependent and 10 H pylori-independent cases. CD86 expression was detected in 11 (68.8%) of 16 H pylori-dependent cases but in none of 10 H pylori-independent cases (P = 0.001). H pylori-dependent high-grade gastric MALT lymphomas contained significantly higher numbers of CD56 (+) NK cells than H pylori-independent cases (2.8±1.4% vs 1.10.8%; P = 0.003). CD86 positive MALT lymphomas also showed significantly increased infiltration of CD56 (+) NK cells compared to CD86-negative cases (2.9±1.1% vs 1.4±1.3%; P = 0.005).

CONCLUSION: These results suggest that the expression of co-stimulatory marker CD86 and the increased infiltration of NK cells are associated with H pylori-dependent state of early-stage high-grade gastric MALT lymphomas.

Keywords: MALT lymphoma; H pylori; CD86; CD56