Co-expression of heat shock protein 70 and glucose-regulated protein 94 in human gastric carcinoma cell line BGC-823

doi:10.3748/wjg.v11.i23.3601

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 21, 2005; 11(23): 3601-3604
Published online Jun 21, 2005. doi: 10.3748/wjg.v11.i23.3601

Co-expression of heat shock protein 70 and glucose-regulated protein 94 in human gastric carcinoma cell line BGC-823

Xiao-Ping Wang, Jing Liao, Guo-Zhen Liu, Xing-Cui Wang, Hong-Wei Shang

Xiao-Ping Wang, Jing Liao, Guo-Zhen Liu, Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China

Xing-Cui Wang, Hong-Wei Shang, Laboratory of Morphology, Capital University of Medical Sciences, Beijing 100054, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Xiao-Ping Wang, Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China. wxpphd@yahoo.com.cn

Telephone: +86-10-63051455

Received: June 30, 2004
Revised: July 1, 2004
Accepted: July 11, 2004
Published online: June 21, 2005

Abstract

AIM: To investigate the co-expression and significance of heat shock protein 70 (HSP70) and glucose-regulated protein 94 (grp94) in human gastric carcinoma cell line BGC-823.

METHODS: The expression and localization of HSP70 and grp94 in human gastric carcinoma cell line BGC-823 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. Flow cytometry was used to analyze the correlation between expression of HSP70, grp94 and cell cycle in BGC-823 cell line.

RESULTS: Gastric cancer cell line BGC-823 expressed high level of HSP70 and grp94. The positive rate of HSP70 and grp94 was 84.9±4.94% and 79.6±5.16%, respectively. Both of them were stained in cell plasma. There was a significant difference compared with control group (1.9±0.94%, P<0.01). During the cell cycle, HSP70 and grp94 were continuously expressed in BGC-823.

CONCLUSION: HSP70 and grp94 are highly expressed in human gastric carcinoma BGC-823 cells through the whole cell cycle. There is no relationship between expression of HSP70, grp94 and cell cycle.

Keywords: Heat shock protein 70; Glucose-regulated protein 94; Gastric carcinoma