Peroxynitrite induced decrease in Na+, K+-ATPase activity is restored by taurine

doi:10.3748/wjg.v11.i23.3554

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Jun 21, 2005 (publication date) through Nov 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 21, 2005; 11(23): 3554-3557
Published online Jun 21, 2005. doi: 10.3748/wjg.v11.i23.3554

Peroxynitrite induced decrease in Na⁺, K⁺-ATPase activity is restored by taurine

Necla Kocak-Toker, Murat Giris, Feti Tülübas, Müjdat Uysal, Gülcin Aykac-Toker

Necla Kocak-Toker, Gülcin Aykac-Toker, Müjdat Uysal, Murat Giris, Feti Tülübas, Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa 34093, Istanbul, Turkey

Author contributions: All authors contributed equally to the work.

Correspondence to: Necla Kocak-Toker, Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa 34093, Istanbul, Turkey. nectok@yahoo.com

Telephone: +90-212-4142189-116 Fax: +90-212-4142189-129

Received: July 23, 2004
Revised: July 24, 2004
Accepted: October 13, 2004
Published online: June 21, 2005

Abstract

AIM: Peroxynitrite (ONOO^-) is a powerful oxidant shown to damage membranes. In the present study, the effect of taurine on changes of liver plasma membrane Na⁺, K⁺-ATPase induced by ONOO^- was investigated.

METHODS: Liver plasma membrane was exposed to ONOO^- with or without taurine. Na⁺, K⁺-ATPase activity and lipid peroxidation as thiobarbituric acid reactive substances (TBARS) levels were measured.

RESULTS: Different concentrations of ONOO^- (100, 200, 500, and 1000 μmol/L) were found to decrease liver plasma membrane Na⁺, K⁺-ATPase activity significantly. The depletion of enzyme activity was not concentration dependent. Effects of different concentrations of taurine on liver plasma membrane Na⁺, K⁺-ATPase activity were also measured. Taurine did not cause any increase in enzyme activity. When plasma membranes were treated with 200 μmol/L ONOO^- with different concentrations of taurine, a restoring effect of taurine on enzyme activity was observed. TBARS levels were also measured and taurine was found to decrease the elevated values.

CONCLUSION: Taurine is observed to act as an antioxidant of ONOO^- to decrease lipid peroxidation and thus affect liver plasma membrane Na⁺, K⁺-ATPase by restoring its activity.

Keywords: Na⁺, K⁺-ATPase; Taurine; Peroxynitrite