Brief Reports
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 14, 2005; 11(22): 3485-3489
Published online Jun 14, 2005. doi: 10.3748/wjg.v11.i22.3485
Comparative proteome analysis of untreated and Helicobacter pylori-treated HepG2
Yan Zhang, Xue-Gong Fan, Ren Chen, Zhi-Qiang Xiao, Xue-Ping Feng, Xue-Fei Tian, Zhao-Hui Chen
Yan Zhang, Xue-Gong Fan, Ren Chen, Xue-Fei Tian, Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
Zhi-Qiang Xiao, Xue-Ping Feng, Zhao-Hui Chen, Medical Research Center, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Xue-Gong Fan, Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. xgfan@hotmail.com
Telephone: +86-731-4327392 Fax: +86-731-4327332
Received: July 2, 2004
Revised: July 3, 2004
Accepted: September 30, 2004
Published online: June 14, 2005
Abstract

AIM: To investigate the pathological effect of Helicobacter pylori (H pylori) on human hepatic cells, proteomic methods were used to find and to identify proteins that were overexpressed in HepG2 cells treated by H pylori.

METHODS: H pylori was co-cultured with HepG2 for 6 h. Two-dimensional gel electrophoresis was used to gain the protein expression pattern of untreated and H pylori-treated HepG2. After staining and image analysis, spots of interest were isolated and subjected to mass spectrometry.

RESULTS: Seven proteins, which were up-regulated in H pylori-treated HepG2 cells, were identified. These proteins included integrin beta-1, protein kinase C alpha, LIM/homeobox protein Lhx1, eIF-2-beta, MAP kinase kinase 3, PINCH protein and Ras-related protein Rab-37, which involved in transcription regulation, signal transduction, metabolism and so on.

CONCLUSION: H pylori may exert the pathological effect on HepG2 cells by up-regulating the expression of some proteins.

Keywords: H pylori; HepG2 cells