Genes transactivated by hepatitis C virus core protein, a microarray assay

doi:10.3748/wjg.v11.i22.3351

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Jun 14, 2005; 11(22): 3351-3356
Published online Jun 14, 2005. doi: 10.3748/wjg.v11.i22.3351

Genes transactivated by hepatitis C virus core protein, a microarray assay

Min Liu, Shu-Lin Zhang, Jun Cheng, Yan Liu, Lin Wang, Qing Shao, Jian Zhang, Shu-Mei Lin

Min Liu, Shu-Lin Zhang, Shu-Mei Lin, Department of Infectious Diseases, The First Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China

Jun Cheng, Yan Liu, Lin Wang, Qing Shao, Jian Zhang, Gene Therapy Research Center, Institute of Infectious Diseases, The 302 Hospital of PLA, Beijing 100039, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Min Liu, Department of Infectious Diseases, The First Affilated, Medical College, Xi’anJiaotong University, Xi’an 710061, Shaanxi Province, China. liumin3262@sohu.com

Telephone: +86-10-85323262 Fax: +86-10-85252512

Received: August 31, 2004
Revised: September 1, 2004
Accepted: October 11, 2004
Published online: June 14, 2005

Abstract

AIM: To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection.

METHODS: Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods.

RESULTS: The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method.

CONCLUSION: HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes.

Keywords: Hepatitis C virus; Core protein; Microarray assay