Gastric Cancer
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 7, 2005; 11(21): 3217-3221
Published online Jun 7, 2005. doi: 10.3748/wjg.v11.i21.3217
Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma
Ming-Dong Zhao, Xue-Mei Hu, Dian-Jing Sun, Qun Zhang, Yu-Hao Zhang, Wei Meng
Ming-Dong Zhao, Dian-Jing Sun, Department of Radiology, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China
Xue-Mei Hu, Wei Meng, Department of Immunology of Binzhou Medical College, Binzhou 256603, Shandong Province, China
Qun Zhang, Yu-Hao Zhang, Medical School of Shanghai Jiaotong University, Shanghai 200030, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Xue-Mei Hu, Medical School of Shanghai Jiaotong University, Shanghai 200030, China. xuemeihu@sjtu.edu.cn
Telephone: +86-21-62932910 Fax: +86-21-62832528
Received: December 28, 2003
Revised: December 29, 2003
Accepted: January 8, 2004
Published online: June 7, 2005
Abstract

AIM: To study the effect of IGF-1/IGF-1R and gastrin/CCK-BR on carcinogenesis and development of human gastric carcinoma and to explore its mechanism and provide a credible theoretical foundation for early diagnosis and molecular therapy of gastric carcinoma.

METHODS: mRNA expression levels of IGF-1/IGF-1R and gastrin/CCK-BR were assessed by RT-PCR method in gastric cancer tissues, adjacent mucosa, and tumor-free tissues from 56 patients with gastric carcinoma and normal gastric mucosae from 56 healthy controls. Tissue specimens were obtained by biopsy and confirmed by histological evaluation.

RESULTS: The mRNA levels of IGF-1/IGF-1R were increased in gastric cancer tissues compared with normal tissues from healthy controls and successively increased in tumor-free tissues, adjacent mucosa, and gastric cancer tissues. The mRNA levels of gastrin/CCK-BR were increased in gastric cancer tissues compared with normal tissues from healthy controls. There was a significant difference between gastric cancer tissues and adjacent mucosa and tumor-free tissues, but the mRNA levels of gastrin were not significantly increased in adjacent mucosa and gastric cancer tissues compared with tumor-free tissues. The mRNA levels of CCK-BR were increased in gastric cancer tissues and adjacent mucosa compared with tumor-free tissues, but not significantly increased in adjacent mucosa and gastric cancer tissues compared with gastric cancer tissues.

CONCLUSION: Overexpression of IGF-1/IGF-1R and gastrin/CCK-BR promotes the disorderly proliferation of gastric mucosa epithelia and it is of great significance in the carcinogenesis and development of gastric carcinoma.

Keywords: Gastric carcinoma; mRNA; IGF-1; IGF-1R; Gastrin; CCK-BR; Protein expression