Dynamical changing patterns of glycogen and enzyme histochemical activities in rat liver graft undergoing warm ischemia injury

doi:10.3748/wjg.v11.i17.2662

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May 7, 2005 (publication date) through Nov 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 7, 2005; 11(17): 2662-2665
Published online May 7, 2005. doi: 10.3748/wjg.v11.i17.2662

Dynamical changing patterns of glycogen and enzyme histochemical activities in rat liver graft undergoing warm ischemia injury

Xiao-Shun He, Yi Ma, Lin-Wei Wu, Jin-Lang Wu, Rui-De Hu, Gui-Hua Chen, Jie-Fu Huang

Xiao-Shun He, Yi Ma, Lin-Wei Wu, Gui-Hua Chen, Jie-Fu Huang, Organ Transplantation Center, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China

Jin-Lang Wu, Rui-De Hu, Department of Pathology, Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Xiao-Shun He, Organ Transplantation Center, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China. xshe@gdvnet.com

Telephone: +86-20-87334322

Received: March 3, 2004
Revised: March 4, 2004
Accepted: June 7, 2004
Published online: May 7, 2005

Abstract

AIM: To investigate the changing patterns of glycogen and enzyme histochemical activities in rat liver graft under a different warm ischemia time (WIT) and to predict the tolerant time limitation of the liver graft to warm ischemia injury.

METHODS: The rats were randomized into five groups, WIT was 0, 15, 30, 45, 60 min, respectively, and histochemical staining of liver graft specimens was observed. The recovery changes of glycogen and enzyme histochemistry activities were measured respectively 6 and 24 h following liver graft implantation.

RESULTS: The activities of succinic dehydrogenase, cytochrome oxidase, apyrase (Mg++-ATPase) and content of glycogen were decreased gradually after different WIT in a time-dependent manner. The changes were significant when WIT was over 30 min.

CONCLUSION: Hepatic injury is reversible within 30 min of warm ischemia injury. Glycogen and enzyme histochemistry activities of liver grafts and their recovery potency after reperfusion may serve as criteria to evaluate the quality of liver grafts.

Keywords: Liver transplantation; Warm ischemia injury; Histochemical stain