Gastric Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 1, 2004; 10(9): 1256-1261
Published online May 1, 2004. doi: 10.3748/wjg.v10.i9.1256
Association of tumor necrosis factor genetic polymorphism with chronic atrophic gastritis and gastric adenocarcinoma in Chinese Han population
Bao-Ying Fei, Bing Xia, Chang-Sheng Deng, Xiao-Qing Xia, Min Xie, J Bart A Crusius, A Salvador Pena
Bao-Ying Fei, Department of Gastroenterology, Zhejiang Provincial People’s Hospital, Hangzhou 310014, Zhejiang Province, China
Bing Xia, Chang-Sheng Deng, Department of Gastroenterology, Wuhan University Zhongnan Hospital, Wuhan 430071, Hubei Province, China
Xiao-Qing Xia, Min Xie, Department of Tumor Surgery, Hubei Provincial Tumor Hospital, Wuhan 430071, Hubei Province, China
J Bart A Crusius, A Salvador Pena, Laboratory of GI immunogenetics Vrije Universiteit Medical Center, Amsterdam, The Netherlands
Author contributions: All authors contributed equally to the work.
Supported by Grant from provincial public health bureau, Hubei Province, No. 97420
Correspondence to: Dr. Bao-Ying Fei, Department of Gastroenterology, Zhejiang Provincial People’s Hospital, Hanzhou 310014, Zhejiang Province, China. feibaoying6924@tom.com
Telephone: +86-571-85239988 Fax: +86-571-85131448
Received: November 18, 2003
Revised: December 8, 2003
Accepted: December 16, 2003
Published online: May 1, 2004
Abstract

AIM: To investigate the association of TNF polymorphisms with chronic atrophic gastritis (CAG) and gastric adenocarcin-oma in Chinese Han patients.

METHODS: The TNFa-e 5 microsatellites and 3 RFLP sites were typed using PCR technique, followed by high-voltage denaturing PAGE with silver staining and restriction enzyme digestion respectively in specimens from 53 patients with CAG and 56 patients with gastric adenocarcinoma and 164 healthy controls. The PCR products were cloned and sequenced.

RESULTS: The frequency of TNF-β Ncol*1/2 genotype was higher in patients with chronic atrophic gastritis than in healthy controls, but no significant difference was observed (60.38% vs 46.34%, P = 0.076). The frequency of TNa10 allele was significantly higher in patients with chronic atrophic gastritis than in healthy controls (19.81% vs 11.89%, P = 0.04). However, it did not relate to age, gender, atrophic degree or intestinal metaplasia in patients with chronic atrophic gastritis. The frequency of TNF-β Ncol*1/2 and d2/d6 genotypes were significantly higher in patients with gastric adenocarcinoma than in healthy individuals (P > 0.05). However, TNF-β Ncol*1/2 and d2/d6 genotypes did not relate to age, gender, grade of differentiation and clinicopathologic stage in patients with gastric adenocarcinoma. The frequency of TNFa6b5c1 haplotype homozygote was significantly lower in patients with gastric adenocarcinoma than in healthy controls (1.79% vs 15.85%, P = 0.006).

CONCLUSION: TNFa10 allele may be a risk factor for chronic atrophic gastritis. TNF-β Ncol*1/2 and d2/d6 genotypes are associated with the susceptibility to gastric adenocarcinoma, whereas TNFa6b5c1 haplotype homozygote may contribute to the resistance against gastric adenocarcinoma.

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