Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 1, 2004; 10(7): 1075-1077
Published online Apr 1, 2004. doi: 10.3748/wjg.v10.i7.1075
New mutation points in 23S rRNA gene associated with Helicobacter pylori resistance to clarithromycin in northeast China
Qing Hao, Yan Li, Zhi-Jie Zhang, Yong Liu, Hong Gao
Qing Hao, Yan Li, Department of Gastroenterology, the 2nd Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Zhi-Jie Zhang, Yong Liu, Clinical Center of Microbiology, the 2nd Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Hong Gao, The Major Health Ministry Lab For Congenital Malformation, China Medical University, Shenyang 110004, Liaoning Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Yan Li, Department of Gastroenterology, the 2nd Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. liyan1@medmail.com.cn Telephone: +86-24-83956416 Fax: +86-24-83250146
Received: October 20, 2003
Revised: November 12, 2003
Accepted: December 16, 2003
Published online: April 1, 2004
Abstract

AIM: To investigate the resistance rate of Helicobacter pylori (H pylori ) to clarithromycin, metronidazole, amoxicillin and tetracycline to guide clinical practice, and to study the mechanism of H pylori resistant to clarithromycin.

METHODS: Thirty H pylori strains were isolated from the mucosa of peptic ulcer, gastric tumor and chronic gastritis patients, then the minimal inhibitory concentration (MIC) to clarithromycin, metronidazole, amoxicillin and tetracycline was evaluated by E-test method. The sequence analysis of PCR fragments was conducted in 23S rRNA gene of H pylori resistant to clarithromycin to get the resistance mechanism of the bacteria.

RESULTS: Among 30 H pylori strains, 7 cases were resistant to clarithromycin, 12 to metronidazole, 2 to tetracycline and no strain was found to be resistant to amoxicillin. The resistance rates were 23.3%, 40%, 6.7% and 0%, respectively. Three new mutation points were found to be related to the clarithromycin resistance in H pylori isolates, which were G2224A, C2245T and T2289C.

CONCLUSION: In northeast China, H pylori shows high resistance to metronidazole, while sensitive to amoxicillin. The mechanism of resistance to clarithromycin may be related to the mutation of G2224A, C2245T and T2289C in the 23S rRNA gene.

Keywords: $[Keywords]