Liver Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 15, 2004; 10(6): 809-812
Published online Mar 15, 2004. doi: 10.3748/wjg.v10.i6.809
Effects of PI3K and p42/p44 MAPK on overexpression of vascular endothelial growth factor in hepatocellular carcinoma
Geng-Wen Huang, Lian-Yue Yang, Wei-Qun Lu
Geng-Wen Huang, Lian-Yue Yang, Wei-Qun Lu, Department of Surgery and Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the grant from National Key Technologies R and D Program, No.2001BA703B04 and the grant from Hunan Province Developing Planning Committee, No.2001-907
Correspondence to: Lian-Yue Yang, MD. Department of Surgery and Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. lianyueyang@hotmail.com
Telephone: +86-731-4327326
Received: November 18, 2003
Revised: December 2, 2003
Accepted: December 8, 2003
Published online: March 15, 2004
Abstract

AIM: To study the relationship between hypoxia or epidermal growth factor (EGF) and the overexpression of vascular endothelial growth factor (VEGF) in hepatocellular carcinoma (HCC) and the signal transduction pathway of the transcription of VEGF in hepatoma cells.

METHODS: Cobalt chloride and recombinant human EGF were used to stimulate the hepatoma cell lines HepG2. VEGF mRNA was detected by using of semi-quantitative polymerase chain reaction (RT-PCR). Specific inhibitors of phosphatidylinositol 3-kinase (PI3K) and p42/p44 mitogen activated protein kinase (MAPK) were used to observe the effects of the two kinases on the regulation of the transcription of VEGF in hepatoma cells.

RESULTS: The expression of VEGF mRNA in HepG2 cells cultured in serum-free medium was 0.117. However, 100 μmol/L cobalt chloride for 24 h increased the expression of VEGF mRNA and VEGF mRNA increased gradually with the increase of the concentration and duration of cobalt chloride. Also, 25 ng/mL recombinant human EGF stimulated the expression of VEGF in HepG2 cells and the expression increased with the increase of EGF concentration. 5 μmol/L LY294002 inhibited the expression of VEGF stimulated by cobalt chloride or recombinant human EGF and the inhibition decreased step by step with increase of the concentration of LY294002. But even 20 μmol/L LY294002 could not completely block the expression of VEGF. In contrast, PD98059 had no inhibitory effects on the transcription of VEGF stimulated by cobalt chloride or recombinant human EGF.

CONCLUSION: The overexpression of VEGF in HCC could be promoted by hypoxia and EGF expression in HCC. The signal transduction pathway of VEGF transcription in HepG2 cells may be through PI3K pathway, but not through p42/p44 MAPK pathway.

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