Gastric Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 1, 2004; 10(3): 348-351
Published online Feb 1, 2004. doi: 10.3748/wjg.v10.i3.348
Apoptosis of human gastric adenocarcinoma cells induced by β-ionone
Jia-Ren Liu, Bing-Qing Chen, Bao-Feng Yang, Hong-Wei Dong, Chang-Hao Sun, Qi Wang, Guo Song, You-Qiang Song
Jia-Ren Liu, Bing-Qing Chen, Hong-Wei Dong, Chang-Hao Sun, Qi Wang, Public Health College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Bao-Feng Yang, Guo Song, Department of Pharmacology, Harbin Medical University, Harbin 150086, Heilongjiang Province, China You-Qiang Song, Department of Biochemistry and The Genome Research Center, The University of Hong Kong, Hong Kong, China
Author contributions: All authors contributed equally to the work.
Supported by The National Natural Science Foundation of China, No. 30200229 and The Postdoctoral Foundations of China and Heilongjiang Province, China
Correspondence to: Dr. Bao -Feng Yang, Department of Pharmacology, Harbin Medical University, Harbin 150086, Heilongjiang Province, China. jiarl@ems.hrbmu.edu.cn Telephone: +86-451-53641309 Fax: +86-451-53648617
Received: August 26, 2003
Revised: October 5, 2003
Accepted: October 12, 2003
Published online: February 1, 2004
Abstract

AIM: To investigate the effect of β-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901.

METHODS: Using MTT, fluorescence dye (Hoechst-33258), transmission electron microscopy and the TUNEL assay, we examined growth and apoptosis of SGC-7901 cells treated with β-ionone at various concentrations (i.e. 25, 50, 100 and 200 μmol/L) for 24 h, 48 h.

RESULTS: The growth of SGC-7901 cells was inhibited by β-ionone. Seven days after treatment with β-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%, 78.25% and 94.15%, respectively. The IC50 value of β-ionone for SGC-7901 cells was estimated to be 89 μmol/L. The apoptotic morphology was demonstrated in SGC-7901 cells treated with β-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in β-ionone-treated SGC-7901 cells by the TUNEL assay.

CONCLUSION: β-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells. However, the mechanism needs to be further investigated.

Keywords: $[Keywords]