Effect of hepatoma H22 on lymphatic endothelium in vitro

doi:10.3748/wjg.v10.i23.3428

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Dec 1, 2004 (publication date) through Nov 24, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Liver Cancer

World J Gastroenterol. Dec 1, 2004; 10(23): 3428-3432
Published online Dec 1, 2004. doi: 10.3748/wjg.v10.i23.3428

Effect of hepatoma H22 on lymphatic endothelium in vitro

Hua Yu, Hong-Zhi Zhou, Chun-Mei Wang, Xiao-Ming Gu, Bo-Rong Pan

Hua Yu, Chun-Mei Wang, Electron Microscopy Center, School of Basic Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Hong-Zhi Zhou, Xiao-Ming Gu, College of Stomatology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Bo-Rong Pan, Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Chun-Mei Wang, Electron Microscopy Center, School of Basic Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. wangcm@fmmu.edu.cn

Telephone: +86-29-83374569 Fax: +86-29-83374467

Received: April 14, 2004
Revised: April 19, 2004
Accepted: May 9, 2004
Published online: December 1, 2004

Abstract

AIM: To determine the effect of metastatic hepatoma cells on lymphangioma-derived endothelium, and to establish in vitro model systems for assessing metastasis-related response of lymphatic endothelium.

METHODS: Benign lymphangioma, induced by intraperitoneal injection of the incomplete Freund’s adjuvant in BALB/c mice, was embedded in fibrin gel or digested and then cultured in the conditioned medium derived from hepatoma H22. Light and electron microscopy, and the transwell migration assay were used to determine the effect of H22 on tissue or cell culture. Expressions of Flt-4, c-Fos, proliferating cell nuclear antigen (PCNA), and inducible nitric oxide synthase (iNOS) in cultured cells, and content of nitric oxide in culture medium were also examined.

RESULTS: The embedded lymphangioma pieces gave rise to array of capillaries, while separated cells from lymphangioma grew to a cobblestone-like monolayer. H22 activated growth and migration of the capillaries and cells, induced expressions of Flt-4, c-Fos, PCNA and iNOS in cultured cells, and significantly increased the content of NO in the culture medium.

CONCLUSION: Lymphangioma-derived cells keep the differentiated phenotypes of lymphatic endothelium, and the models established in this study are feasible for in vitro study of metastasis-related response of lymphatic endothelium.

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