Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 15, 2004; 10(22): 3353-3355
Published online Nov 15, 2004. doi: 10.3748/wjg.v10.i22.3353
Protective effect of rhIL-1β on pancreatic islets of alloxan-induced diabetic rats
Li-Ping Wu, Li-Hua Chen, Jin-Shan Zhang, Lan Sun, Yuan-Qiang Zhang
Li-Ping Wu, the Second Department of Geriatrics and Gerontology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Li-Hua Chen, Department of Immunology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Jin-Shan Zhang, Lan Sun, Yuan-Qiang Zhang, Department of Histology and Embryology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 39870109
Correspondence to: Professor Yuan-Qiang Zhang, Department of Histology and Embryology, the Fourth Military Medical University, 169 Changle West Road, Xi’an 710032, Shaanxi Province, China. zhangyq@fmmu.edu.cn
Telephone: +86-29-83374508 Fax: +86-29-83374508
Received: November 4, 2003
Revised: December 4, 2003
Accepted: December 8, 2003
Published online: November 15, 2004
Abstract

AIM: To observe the protective effect of rhIL-1β on pancreatic islets of alloxan-induced diabetic rats.

METHODS: Protection of rhIL-1β on pancreatic islets of alloxan-induced diabetic rats (n = 5) was demonstrated with methods of immunohistochemistry and stereology. The concentration of serum glucose was measured by GOD method and that of serum insulin by RIA.

RESULTS: The concentration of serum glucose increased but that of insulin decreased after administration of alloxan(150 mg/kg), and the volume density and numerical density of the islets were zero. In rhIL-1β pretreated rats, although the concentration of serum insulin decreased (from 11.9 ± 3.0 mIU/L to 6.1 ± 1.6 mIU/L, P < 0.05), that of glucose was at normal level compared with the control group. As compared with alloxan group, the concentration of serum glucose in rhIL-1β pretreated rats decreased (from 19.4 ± 8.9 mmol/L to 12.0 ± 4.0 mmol/L, P < 0.05) and the volume density increased(0/L to. 1/L, P < 0.05).

CONCLUSION: rhIL-1β pretreatment may have protective effect on the islets of alloxan-induced diabetic rats.

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