Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 1, 2004; 10(19): 2883-2885
Published online Oct 1, 2004. doi: 10.3748/wjg.v10.i19.2883
Quantifying anti-HBV effect of targeted ribonuclease by real-time fluorescent PCR
Jun Liu, Ying-Hui Li, Jin Ding, Wei-Dong Gong, Cai-Fang Xue, Ya Zhao, Yu-Xiao Huang
Jun Liu, Ying-Hui Li, Jin Ding, Wei-Dong Gong, Cai-Fang Xue, Ya Zhao, Yu-Xiao Huang, Department of Etiology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30100157; Medical Research Fund of Chinese PLA, No. 01MA184; Innovation Project of FMMU, No. CX99005
Correspondence to: Dr. Jun Liu, Department of Etiology, Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China. etiology@fmmu.edu.cn
Telephone: +86-29-83374536 Fax: +86-29-83374594
Received: March 11, 2004
Revised: April 6, 2004
Accepted: April 13, 2004
Published online: October 1, 2004
Abstract

AIM: To quantify the inhibition of HBV replication by targeted ribonuclease by using real-time fluorescent PCR.

METHODS: Targeted ribonuclease was introduced into 2.2.15 cells by liposome-mediated transfection or HIV-TAT mediated protein transduction. Forty-eight hours after the transfection and 24 h after the transduction, supernatants of 2.2.15 cells were collected and HBV DNA in the supernatants was quantified by real-time fluorescent PCR with a commercial kit.

RESULTS: HBV DNA concentrations in the supernatants of 2.2.15 cells transfected or transducted with targeted ribonuclease were 4.9 ± 2.4 × 108 copies/L and 8.3 ± 4.0 × 108 copies/L, respectively. Compared with controls, transfection or transduction of targeted ribonuclease reduced HBV DNA concentration in the supernatants of 2.2.15 cells by 90.4% and 90.1%, respectively (P < 0.05).

CONCLUSION: Targeted ribonuclease can inhibit HBV replication in 2.2.15 cells.

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