H Pylori
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 1, 2004; 10(17): 2493-2497
Published online Sep 1, 2004. doi: 10.3748/wjg.v10.i17.2493
Helicobacter pylori cagA, iceA and vacA genotypes in patients with gastric cancer in Taiwan
Hwai-Jeng Lin, Chin-Lin Perng, Wen-Ching Lo, Chew-Wun Wu, Guan-Ying Tseng, Anna Fen-Yau Li, I-Chen Sun, Yueh-Hsing Ou
Hwai-Jeng Lin, I-Chen Sun, Division of Gastroenterology, Department of Medicine, VGH-TAIPEI, Taiwan, China
Chin-Lin Perng, I-Lan Hospital, Department of health, Taiwan, China
Wen-Ching Lo, Zhongxiao Municipal Hospital, Taipei, Taiwan, China
Chew-Wun Wu, Division of General Surgery, Department of Surgery, VGH-TAIPEI, Taiwan, China
Guan-Ying Tseng, Ton-Yen General Hospital, Hsin-Chu, Taiwan, China
Anna Fen-Yau Li, Department of Pathology, VGH-TAIPEI, Taiwan, China
Yueh-Hsing Ou, Institute of Biotechnology in Medicine, School of Medical Technology and Engineering, and School of Medicine, National Yang-Ming University, Taiwan, China
Author contributions: All authors contributed equally to the work.
Supported by VGH 91-274, NSC 91-2314-B075-127
Correspondence to: Professor Hwai-Jeng Lin, Division of Gastroenterology, Department of Medicine, VGH-TAIPEI, Shih-Pai Rd, Sec 2, Taipei, 11217, Taiwan, China. hjlin@vghtpe.gov.tw
Telephone: +886-2-28712121 Ext. 2015 Fax: +886-2-28739318
Received: November 12, 2003
Revised: December 4, 2003
Accepted: December 15, 2003
Published online: September 1, 2004
Abstract

AIM: Helicobacter pylori (H pylori) has been linked to chronic gastritis, peptic ulcer, gastric cancer and MALT-lymphoma. The link of genotypes of H pylori to gastric cancer remains controversial. The aim of this study was to investigate the H pylori vacA alleles, cagA and iceA in patients with gastric cancer in Taiwan.

METHODS: Patients with gastric cancer, peptic ulcer and chronic gastritis were enrolled in this study. We obtained biopsy specimens from the stomach at least 2 cm away from the tumor margin in patients with gastric cancer, and from the antrum of stomach in patients with peptic ulcer or chronic gastritis. DNA extraction and polymerase chain reaction were used to detect the presence or absence of cagA and to assess the polymorphism of vacA and iceA.

RESULTS: A total of 168 patients (gastric ulcer: 77, duodenal ulcer: 66, and chronic gastritis: 25) were found to have positive PCR results of the biopsy specimens from patients with peptic ulcer and chronic gastritis. We found positive cagA (139/168, 83%), m2 (84/168, 50%) and iceA1 (125/168, 74%) strains in the majority of patients. In patients with gastric cancer, the vacA s1a and s1c subtypes were less commonly found than those in non-cancer patients (35/66 vs 127/168, P = 0.0001 for s1a and 13/66 vs 93/168, P < 0.0001 for s1c). In the middle region, the m1T strain in patients with gastric cancer was more than that of non-cancer patients (23/66 vs 33/168, P = 0.02).

CONCLUSION: In Taiwan, H pylori with positive vacA s1a, cagA and iceA1 strains are found in the majority of patients with gastric cancer or non-cancer patients. In patients with gastric cancer, the vacA s1a and s1c subtypes are less and m1T is more than in patients with peptic ulcer and chronic gastritis.

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