Viral Hepatitis
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 2004; 10(16): 2327-2329
Published online Aug 15, 2004. doi: 10.3748/wjg.v10.i16.2327
New serum biomarkers for detection of HBV-induced liver cirrhosis using SELDI protein chip technology
Xiao-Dong Zhu, Wei-Hua Zhang, Cheng-Lin Li, Yang Xu, Wei-Jiang Liang, Po Tien
Xiao-Dong Zhu, Wei-Jiang Liang, Po Tien, Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
Wei-Hua Zhang, Yang Xu, Ciphergen Biosystems, Inc., Beijing, China
Cheng-Lin Li, Department of Pathology, Beijing You’an Hospital, Beijing 100054, China
Author contributions: All authors contributed equally to the work.
Supported by the Major State Basic Research Development Program of China (973 Program), No. 2001CB510001
Correspondence to: Professor Po Tien, Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Zhongguancun Beiyitiao, Beijing 100080, China. tienpo@sun.im.ac.cn
Telephone: +86-10-62554247 Fax: +86-10-62622101
Received: December 12, 2003
Revised: January 2, 2004
Accepted: February 18, 2004
Published online: August 15, 2004
Abstract

AIM: To find new serum biomarkers for liver cirrhosis (LC) in chronic carriers of hepatitis B virus (HBV).

METHODS: Surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry was used to discover biomarkers for differentiating HBV induced LC from non-cirrhotic cohorts. A training population of 25 patients with HBV-induced LC, 20 patients with HCC, and 25 closely age-matched healthy men, was studied.

RESULTS: Two biomarkers with Mr 7772 and 3933 were detected in sera of non-cirrhotic cohorts, but not in patients with HBV-induced LC. A sensitivity of 80% for all LC patients, a specificity of 81.8% for all non-cirrhotic cohorts and a positive predictive value of 75% for the study population were obtained.

CONCLUSION: These two serum biomarkers for HBV-induced LC might be used for diagnosis and assessment of disease progression.

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