H Pylori
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 15, 2004; 10(14): 2060-2062
Published online Jul 15, 2004. doi: 10.3748/wjg.v10.i14.2060
Gene distribution of cagII in Helicobacter pylori-infected patients of Zhejiang Province
Hai-Yan Liu, Ping-Chu Fang, Yun-Shui Jiang, Ran Tao, Jin Chen
Hai-Yan Liu, Ping-Chu Fang, Yun-Shui Jiang, Ran Tao, Jin Chen, Department of Medical Microbiology and Parasitology, School of Medicine, Zhejiang University, Hangzhou 310031, Zhejiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Project of China Medical Board, No.96-628, and the Natural Science Foundation of Zhejiang Province, No.302023
Correspondence to: Ping-Chu Fang, Department of Medical Microbiology and Parasitology, School of Medicine, Zhejiang University, 353 Yanan Road, Hangzhou 310031, Zhejiang Province, China. fangpc@cmm.zju.edu.cn
Telephone: +86-571-87217403
Received: December 19, 2003
Revised: December 28, 2003
Accepted: January 15, 2004
Published online: July 15, 2004
Abstract

AIM: To determine the prevalence of genotypes of cagII in Helicobacter pylori (H pylori)-infected patients in Zhejiang Province and investigate the relationship between these genotypes and the types of gastroduodenal diseases.

METHODS: One hundred and seventy one clinical isolates were collected from 70 chronic superficial gastritis, 31 chronic atrophic gastritis, 41 gastric ulcer, 21 duodenal ulcer, 3 gastric and duodenal ulcer, and 5 gastric adenocarcinoma patients. Polymerase chain reaction assays were performed for analysis of cagT, ORF13 and ORF10 genes in the cagII region.

RESULTS: Of 171 H pylori isolates from Zhejiang patients, 159 (93.0%) were positive for all the three loci. One isolate (0.6%) was negative for all the three loci, and 11 (6.4%) were partially deleted in cagII. The positive rates of cagT, ORF13 and ORF10 genes were 97.1%, 94.7% and 99.4%, respectively. In the strains isolated from the patients with diseases including chronic superficial gastritis, chronic atrophic gastritis, gastric ulcer and duodenal ulcer, the sitive rates of cagT were 95.7%, 100.0%, 95.1% and 100.0%, respectively. The positive rates of ORF13 were 94.3%, 93.5%, 95.1% and 100.0%, respectively. The sitive rates of ORF10 were 98.6%, 100.0%, 100.0% and 100.0%, respectively. The three genes were all positive in the three H pylori strains isolated from the patients with both gastric and duodenal ulcer. In the five strains isolated from the patients with gastric adenocarcinoma, only one isolate was negative for ORF13. There were no significant differences of the cagT, ORF13 and ORF10 genes among the different gastroduodenal diseases including chronic superficial gastritis, chronic atrophic gastritis, gastric ulcer, duodenal ulcer, both gastric and duodenal ulcer and gastric adenocarcinoma (χ2 = 3.098, P > 0.05 for cagT; χ2 = 3.935, P > 0.05 for ORF13 and χ2 = 6.328, P > 0.05 for ORF10).

CONCLUSION: The cagII is not a uniform and conserved entity. Although the genes in cagII are highly associated with the gastroduodenal diseases, the clinical outcome of H pylori infection is not reliably predicted by the three genes in cagII in patients from Zhejiang Province.

Keywords: $[Keywords]