Yu JC, Sun KL, Liu B, Fu SB. Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer. World J Gastroenterol 2004; 10(13): 1964-1966 [PMID: 15222047 DOI: 10.3748/wjg.v10.i13.1964]
Corresponding Author of This Article
Dr. Song-Bin Fu, Laboratory of Medical Genetics, Harbin Medical University, Harbin 150086, Heilongjiang Province, China. fusb@ems.hrbmu.edu.cn
Article-Type of This Article
Brief Reports
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Jul 1, 2004; 10(13): 1964-1966 Published online Jul 1, 2004. doi: 10.3748/wjg.v10.i13.1964
Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer
Jing-Cui Yu, Kai-Lai Sun, Buo Liu, Song-Bin Fu
Jing-Cui Yu, Department of Medical Genetics, China Medical University, Shenyang 110001, China; Department of Clinical Pharmacology, the Second Affiliated Hosptial, Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Song-Bin Fu, Laboratory of Medical Genetics, Harbin Medical University; Bio-pharmaceutical Key Laboratory of Heilongjiang Province, Harbin 150086, Heilongjiang Province, China
Kai-Lai Sun, Department of Medical Genetics, China Medical University, Shenyang 110001, Liaoning Province, China
Buo Liu, Chinese National Human Genome Center, Beijing 100176, China
Author contributions: All authors contributed equally to the work.
Supported by the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions by Ministry of Education of China and the National Natural Science Foundation, No. 30370783 and the Key Project of Science and Technology of Heilongjiang Province, No. GB03C601-1
Correspondence to: Dr. Song-Bin Fu, Laboratory of Medical Genetics, Harbin Medical University, Harbin 150086, Heilongjiang Province, China. fusb@ems.hrbmu.edu.cn
Telephone: +86-451-86674798 Fax: +86-451-86669576
Received: December 23, 2003 Revised: January 22, 2004 Accepted: February 1, 2004 Published online: July 1, 2004
Abstract
AIM: To investigate the association between loss of heterozygosity (LOH) on chromosome 18 and sporadic gastric cancer.
METHODS: Multiplex PCR was used to screen 14 highly polymorphic microsatellite markers on chromosome 18 in 45 cases of primary gastric cancer. PCR products were separated on polyacrylamide gels and the electrophoresis maps were analyzed with Genescan and Genotyper.
RESULTS: The LOH frequencies in gastric cancer at all 14 markers ranged from 10% to 58%. Eleven markers were found with over 20% LOH frequencies, in which 9 markers located in 18q, and 2 markers in 18p. Two overlapping deleted regions were identified: R1 between D18S61-D18S1161 at 18q22 (9 cM) with 24% LOH frequency; R2 between D18S462-D18S70 at 18q22-23 (6 cM) with 32% LOH frequency.
CONCLUSION: LOH of chromosome 18 (18q and 18p) may be involved in gastric tumorigenesis. Two overlapping deleted fragments suggested that there might be unidentified tumor suppressor genes in those two regions.
