Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 15, 2004; 10(10): 1495-1498
Published online May 15, 2004. doi: 10.3748/wjg.v10.i10.1495
Synthesis of ribozyme against vascular endothelial growth factor165 and its biological activity in vitro
Zhong-Ping Gu, Yun-Jie Wang, Yu Wu, Jin-Ge Li, Nong-An Chen
Zhong-Ping Gu, Yun-Jie Wang, Yu Wu, Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China
Jin-Ge Li, Department of Infectious Disease, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China
Nong-An Chen, Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 20020, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Zhong-ping Gu, Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China. zhongpg@xaonline.com.cn
Telephone: +86-29-3541718
Received: December 10, 2003
Revised: January 24, 2004
Accepted: February 1, 2004
Published online: May 15, 2004
Abstract

AIM: To investigate the designation, synthesis and biological activity of against vascular endothelial growth factor165 (VEGF165) ribozyme.

METHODS: The ribozyme against VEGF165 was designed with computer. The transcriptional vector was constructed which included the anti-VEGF165 ribozyme and 5’, 3’ self-splicing ribozymes. The hammerhead ribozyme and substrate VEGF165 mRNA were synthesized through transcription in vitro. The cleavage activity of the ribozyme on target RNA was observed in a cell-free system.

RESULTS: The anti-VEGF165 ribozyme was released properly from the transcription of pGEMRz212 cleaved by 5’ and 3’ self-splicing ribozymes which retained its catalytic activity, and the cleavage efficiency of ribozyme reached 90.7%.

CONCLUSION: The anti-VEGF165 ribozyme designed with computer can cleave VEGF165 mRNA effectively.

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