Gastric Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 15, 2004; 10(10): 1392-1396
Published online May 15, 2004. doi: 10.3748/wjg.v10.i10.1392
Effects of c9,t11-conjugated linoleic acid on adhesion of human gastric carcinoma cell line SGC-7901
Bing-Qing Chen, Yan-Mei Yang, Qi Wang, Yan-Hui Gao, Jia-Ren Liu, Jing-Shu Zhang, Xuan-Lin Wang, Rui-Hai Liu
Bing-Qing Chen, Qi Wang, Jia-Ren Liu, Jing-Shu Zhang, Xuan-Lin Wang, Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Yan-Mei Yang, Medical College of Shantou University, Shantou 515031, Guangdong Province, China
Yan-Hui Gao, Chinese Center for Disease Control and Prevention, the Center for Endemic Disease Control, Beijing, China
Rui-Hai Liu, Food Science and Toxicology, Department of Food Science, 108 Stocking Hall, Cornell University, Ithaca, NY 14853-7201, USA
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30070658
Correspondence to: Professor Bing-Qing Chen, Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China. bingqingchen@sina.com
Telephone: +86-451-3608014 Fax: +86-451-3648617
Received: October 8, 2003
Revised: December 1, 2003
Accepted: December 8, 2003
Published online: May 15, 2004
Abstract

AIM: To investigate the effect of c9,t11-conjugated linoleic acid (c9, t11-CLA) on the adhesion of human gastric carcinoma cell line (SGC-7901).

METHODS: SGC-7901 cells were at first treated with different concentrations (25, 50, 100, 200 μmol/L) of c9, t11-CLA and 1 mL/L ethanol (as a negative control) for 24 h. Using adhesion assay and Western blot, we investigated the ability of SGC-7901 cells to adhere to intracellular matrix and examined the expression of E-cadherin (ECD), α-catenin, intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in these cells.

RESULTS: The attachment rate to laminin of SGC-7901 cells treated with different concentrations of c9, t11-CLA (0, 25, 50, 100, and 200 μmol/L) was 100.0 ± 3.3, 95.7 ± 4.0, 89.2 ± 4.6, 87.9 ± 6.1, and 65.9 ± 5.8, respectively. The attachment rate to fibronectin was 100.0 ± 4.7, 96.8 ± 3.8, 94.5 ± 4.1, 76.5 ± 4.3, and 61.8 ± 4.8, respectively. The attachment rate to Matrigel was 99.9 ± 6.6, 91.4 ± 6.8, 85.5 ± 7.4, 79.3 ± 5.6, and 69.6 ± 5.1, respectively. Besides, c9, t11-CLA could increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 in SGC-7901 cells.

CONCLUSION: c9,t11-CLA can reduce the adhesion of human gastric carcinoma cells to laminin, fibronectin and Matrigel. c9,t11-CLA can increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 in human gastric carcinoma cells.

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